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Research in Pharmaceutical Sciences، جلد ۲۰، شماره ۳، صفحات ۴۴۵-۴۵۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Synthesis of thiosemicarbazone derivatives and evaluation of their cytotoxicity with emphasis on ferroptosis biomarkers; an in vitro study |
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| چکیده انگلیسی مقاله |
Background and purpose: This study aimed to evaluate the cytotoxicity of synthesized thiosemicarbazone derivatives, particularly on biomarkers associated with ferroptosis. Experimental approach: Thiosemicarbazone derivatives (C1-C5) were synthesized by condensation between thiosemicarbazide and the corresponding benzaldehyde derivatives. The compounds were characterized using IR spectroscopy and H/C NMR spectroscopy. To evaluate their biological activity, PC-12 cells were cultured in DMEM/MEM medium supplemented with 10% bovine serum albumin. Cell viability was assessed using the MTT assay, while intracellular reactive oxygen species (ROS) levels were measured using DCFH-DA. Additionally, glutathione peroxidase (GPX) activity, lipid peroxidation (LPO), and total antioxidant capacity (TAC) were evaluated to determine oxidative stress and antioxidant response. Findings/Results: In cell viability assessments, C2 exhibited the highest toxicity, while C4 demonstrated the lowest toxicity after 24 h. Among all derivatives, only C3 reduced ROS levels without affecting GPX activity. All derivatives effectively reduced LPO, although C5 showed the least effectiveness in this regard. In contrast to C2 and C5, TAC was significantly higher than the control after treatment with C1, C3, and C4. Conclusion and implications: These findings suggest that thiosemicarbazone derivatives may influence the ferroptosis cell death pathway through their chelation properties, necessitating further research on their ability to bind to iron. Their effects on oxidative stress and cellular antioxidant capacity provide valuable insights for therapeutic strategies. |
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| کلیدواژه‌های انگلیسی مقاله |
Antioxidant capacity,Cytotoxicity,Ferroptosis,Iron chelators,Lipid peroxidation,Thiosemicarbazones. |
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| نویسندگان مقاله |
| Yasaman Shadmani
Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Extremophile and Productive Microorganisms Research Center, Kerman University of Medical Sciences, Kerman, Iran.
| Yaghoub Pourshojaei
Department of Toxicology and Pharmacology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
Pharmaceutics Research Center, Institute of Neuropharmacology, Kerman University of Medical, Kerman, Iran.
| Somayyeh Karami-Mohajeri
Extremophile and Productive Microorganisms Research Center, Kerman University of Medical Sciences, Kerman, Iran.
Department of Pharmaceutical Biotechnology, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
| Bagher Amirheidari
Department of Medicinal Chemistry, Faculty of Pharmacy, Kerman University of Medical Sciences, Kerman, Iran.
| Motahareh Sadeghzadeh
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| نشانی اینترنتی |
http://rps.mui.ac.ir/index.php/jrps/article/view/2317 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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