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Iranian Journal of Microbiology، جلد ۱۷، شماره ۴، صفحات ۵۴۹-۵۵۸
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Exploring the genetic diversity and the association of drug resistance and biofilm production in Acinetobacter baumannii strains isolated from burn wound infections |
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| چکیده انگلیسی مقاله |
Background and Objectives: Acinetobacter baumannii is considered a troublesome cause of infection in burn units, where its capability to form biofilm and resist antibiotics significantly hampers therapeutic success. This study explored the correlations between antimicrobial resistance profiles, biofilm-producing capacity, and genetic diversity of A. baumannii strains from patients with burn wound infection in Isfahan, Iran. Materials and Methods: Ninety-six isolates were analyzed for antibiotic resistance using the disk diffusion technique and for biofilm formation through the microtiter dish assay. The prevalence of ten biofilm-related genes was investigated using specific primers. Clonal relatedness among bacterial strains was defined by Enterobacterial Repetitive Intergenic Consensus-Polymerase Chain Reaction (ERIC-PCR). Results: A vast majority of isolates (99%) exhibited resistance to meropenem, ciprofloxacin, ceftriaxone, cefotaxime, piperacillin-tazobactam, and imipenem, qualifying them as extensively drug-resistant (XDR). Twenty-five percent of the strains were strong biofilm formers, while 68% demonstrated moderate or weak biofilm formation. The most commonly identified biofilm-related genes included bfmR (100%), ompA (100%), and bap (99%). A significant association was found between the production of biofilm, resistance to aminoglycosides, and the presence of csuE and bap genes. ERIC-PCR typing showed the presence of 3 clonal types and 7 single types, with biofilm producers predominantly clustering to clonal type 2. Conclusion: This work highlights a notable prevalence of biofilm-producing XDR A. baumannii in burn patients, underscoring the need for continuous surveillance and enhanced infection control strategies. |
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| نویسندگان مقاله |
| Sanaz Khashei
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
| Hossein Fazeli
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
| Fateh Rahimi
Department of Cell and Molecular Biology & Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, Iran
| Vajihe Karbasizade
Department of Microbiology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, Iran
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| نشانی اینترنتی |
https://ijm.tums.ac.ir/index.php/ijm/article/view/5225 |
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