Veterinary Research Forum، جلد ۱۶، شماره ۷، صفحات ۳۶۵-۳۷۴
عنوان فارسی
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کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Establishment and application of dual isothermal amplification of Pasteurella multocida and Streptococcus suis in pigs
چکیده انگلیسی مقاله Porcine respiratory disease complex is a clinically lethal condition and is the leading cause of mortality in weaned piglets as well as growing and fattening pigs. Pasteurella multocida (Pm) and Streptococcus suis (SS) are common respiratory pathogens in porcine respiratory disease complex. This study combined the recombinase polymerase amplification (RPA) technique with the lateral flow dipstick (LFD) technique. The dual Basic-RPA detection method for Pm and SS and the dual RPA-LFD rapid visualization detection method for Pm and SS were constructed, respectively. The detection limit of RPA-LFD was 10-6 ng μL-1, which was higher than the detection limit of RPA at 10-5 ng μL-1 and much higher than the detection limit of polymerase chain reaction at 10-4 ng μL-1. There was no cross-reactivity with other pathogens which indicated that the method had good specificity and high sensitivity. The detection rate of RPA-LFD was much higher than that of conventional Polymerase chain reaction in 60 clinical samples collected in 2023 with suspected Pm and SS. This method could avoid the complicated temperature cycling instruments and does not require professional laboratory skills, which makes it suitable for on-site detection.
کلیدواژه‌های انگلیسی مقاله Lateral flow dipstick,Partonella multocida,Recombinase polymerase amplification,Streptococcus suis
نویسندگان مقاله Shuang Li |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Jingjing Li |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Kexin Wang |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Qianlei Zhu |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Yafei Chang |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Lei Wang |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Zhanwei Teng |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Xiaobing Wei |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Meinan Chang |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Mingcheng Liu |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Oksana Kasjanenko |
Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine

Sergii Kasianenko |
Faculty of Veterinary Medicine, Sumy National Agrarian University, Sumy, Ukraine

Jianhe Hu |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Huihui Zhang |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

Xiaojing Xia |
College of Animal Science and Veterinary Medicine, Henan Institute of Science and Technology, Xinxiang, China

نشانی اینترنتی https://vrf.iranjournals.ir/article_725432_72ca57d19f0086c5f30ee79977b71c46.pdf
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