| چکیده انگلیسی مقاله |
Background and Objectives: Staphylococcus aureus (S. aureus) is one of the most important pathogens, responsible for a range of infections. This study aimed to assess resistance patterns in S. aureus isolates obtained from certain private-sector laboratories against commonly used antimicrobial agents. Materials and Methods: The process involved collecting various samples from several private laboratories and then identifying S. aureus isolates using biochemical characterization. The antibiotic susceptibility of these isolates was determined by disc diffusion method . Furthermore, Rt-PCR was employed to identify two genes namely the methicillin/oxacillin resistance genes (mecA), and (SCCmec). Results: The findings of the current study exhibited that females constituted a larger proportion of the participants (59.1%) compared to males (40.9%), with a mean participant age of 40.82 years. Gram-positive bacteria were more prevalent (71.3%) than Gram-negative bacteria (18.3%), with S. aureus being the most frequent isolate (60.9%). Urine samples represented the highest collected sample type (47.8%). Out of the 115 bacterial isolates, 85.2% exhibited multidrug resistance to antibiotics such as cefazolin, gentamicin, vancomycin, and ceftazidime. Clindamycin was the most effective antibiotic, with a sensitivity rate of 62.9%, followed by teicoplanin and meropenem, each with a sensitivity rate of 52.9%. Methicillin-resistant Staphylococcus aureus (MRSA) strains were susceptabile to vancomycin and teicoplanin. The methicillin/oxacillin resistant isolates showed significant association with mecA and SCCA genes. Conclusion: This study highlighted the multi-drug resistance in S. aureus isolates, stressing the need for stringent antibiotic stewardship, continuous surveillance, and further research into alternative treatments, including novel antibiotics and combination therapy, to combat resistant strains. |
| نویسندگان مقاله |
| Rania Al-Groom
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan; Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Zarqa, Jordan
| Ghina Al-Saraireh
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan
| Sultan Ayesh Mohammed Saghir
Department of Medical Analysis, Princess Aisha Bint Al-Hussein College of Nursing and Medical Sciences, Al-Hussein Bin Talal University, Ma`an, Jordan
| Mohd Sajjad Ahmad Khan
Department of Basic Sciences, Imam Abdulrahman Bin Faisal University, Dammam, Saudi Arabia
| Areej Almanaseer
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan
| Laila Alswalha
Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Zarqa, Jordan
| Wesal Alraei
Department of Diet Therapy Technology and Dietetics, Faculty of Allied Medical Sciences, Zarqa University, Al-Zarqa, Jordan
| Dalia Abu Al-Haijaa
Department of Diet Therapy Technology and Dietetics, Faculty of Allied Medical Sciences, Zarqa University, Al-Zarqa, Jordan
| Maha Hdaib
Department of Allied Medical Sciences, Zarqa University College, Al-Balqa Applied University, Zarqa, Jordan
| Anas Da'meh
Department of Medical Laboratory Science, Faculty of Allied Medical Sciences, Zarqa University, Zarqa, Jordan
| Shereen Burjaq
Department of Medical Laboratory Sciences, Faculty of Science, Al-Balqa Applied University, Salt, Jordan
| Omar Al-Dmour
Department of Allied Medical Sciences, Zarqa University college, Al-Balqa Applied University, Salt, Jordan
| Fuad Alhawarat
Department of Applied Medical Sciences, Al Hussein Bin Abdullah II Academy for Civil Protection, Al-Balqa Applied University, Salt, Jordan
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