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عنوان انگلیسی Investigating the possible roles of mutations in axin1 and axin2 genes in colorectal cancer
چکیده انگلیسی مقاله Introduction: Colorectal cancer (CRC) is one of the leading cancers, following skin, breast, and stomach cancers. This study aimed to investigate the relationship between mutations in axin1 and axin2 in association with CRC.
Methods: Our study contains 147 fresh frozen samples from CRC patients, 25 normal samples, and 3 cell lines, including HT29, SW480, and CACO-2. The chosen SNPs from databases are placed in exon 5 of axin1, in exon 2 of axin1, and in exon 7 of axin2. By PCR-RFLP method, mutated samples were identified and sequenced.
Results: The results showed that mutations in the single-nucleotide polymorphism (SNP) in axin2 were observed in 1 out of 147 patient samples (0.68%). In the three sequences examined in axin2 (exon 7), mutations in SNP with rs79024445 at A2052C were observed. Statistical analysis of clinical and pathological data of patients showed a significant relationship between the tumor size factor and grade of cancer (P=0.016) as well as the degree of tumor diffusion to the lymph nodes factor with a grade of cancer (P=0.001).
Conclusion: The multi-factorial nature of cancer, the high genetic diversity of the Iranian population, and the limited statistical population could affect these outcomes. The observed mutations in each sample can also indicate the importance of personalized medicine in studying diseases.
کلیدواژه‌های انگلیسی مقاله Axin1,Axin2,SNPs,Colorectal cancer,RFLP
نویسندگان مقاله | Atiye Jalalifar
Department of Molecular Medicine, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.


| Leila Safavi
Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.


| Ramtin Mohammadi
1. Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.2. Medical Biotechnology and Bioinformatics Research Group (MBBRG), Universal Scientific Education And Research Network (USERN), Tehran, Iran.


| Shirin Moradifard
Hudson Institute of Medical Research, Monash University, Melbourne, VIC, Australia


| Mahdieh Banoei
Department of Molecular Medicine, Institute of Medical Biotechnology, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.


| Mohammad Khalilollahi
Department of Biology, North Tehran Branch, Islamic Azad University, Tehran, Iran.


| Shahla Mohammad Ganji
Department of Molecular Medicine, Medical Biotechnology Institute, National Institute of Genetic Engineering and Biotechnology (NIGEB), Tehran, Iran.
نشانی اینترنتی http://mjms.modares.ac.ir/browse.php?a_code=A-10-87937-1&slc_lang=en&sid=30
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