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Journal of Kerman University of Medical Sciences، جلد ۳۱، شماره ۶، صفحات ۲۹۲-۲۹۸
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عنوان انگلیسی Cytotoxic, Antioxidant, and Anti-inflammatory Effects of Crataegus meyeri
چکیده انگلیسی مقاله Background: Crataegus meyeri A. Pojark is one of Iran›s most well-known medicinal plants. This study aimed to assess the cytotoxic, antioxidant, and anti-inflammatory properties of the methanolic extract of Crataegus meyeri (MCM) leaves.
Methods: MCM was prepared from dried leaves. First, the cytotoxic impact of MCM was determined via the MTT test. Then, the anti-inflammatory activity of MCM on the lipopolysaccharide (LPS)-exposed J774A.1 cells was examined by measuring NO production and cyclooxygenase-2 (COX-2) gene expression using the Griess test and quantitative real-time polymerase chain reaction (qRT-PCR), respectively. MCM antioxidant activity was assessed using the FRAP and DPPH tests.
Results: MCM was shown to be non-cytotoxic against J774A.1 cells at≤125 and≤62 μg/mL concentrations after 24 and 48 hours, respectively. It also significantly attenuated NO generation in LPS-treated J774A.1 cells at 62 and 125 μg/mL after 24 hours and 62 μg/mL after 48 hours. The extract significantly reduced COX-2 gene expression at concentrations of 62 and 125 μg/mL after 24 hours and at concentrations of 15, 31, and 62 μg/mL after 48 hours. Moreover, the results indicated that MCM at all concentrations exhibited lower antioxidant activity than vitamin C, but the difference was insignificant. Furthermore, MCM could reduce the viability of most of the studied cancer cell lines at concentrations of 250 and 500 μg/mL.
Conclusion: The findings of the present study suggest that MCM may show therapeutic potential for the treatment of inflammatory diseases, a finding that requires clinical trials.
کلیدواژه‌های انگلیسی مقاله Crataegus meyeri,Murine macrophage J774A.1 cell line,anti-inflammation,Antioxidant,Cytotoxicity
نویسندگان مقاله Mitra Mehrabani |
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

Soheila Mahdavi Yekta |
Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran

Seyed Noureddin Nematollahi-Mahani |
Department of Anatomical Sciences, Afzalipour School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Mehrnaz Mehrabani |
Physiology Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

Arian Amirkhosravi |
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

Mandana Jafari |
Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

Hamed Javdani |
Hormozgan university of medical sciences, Faculty of medicine, Bandar Abbas, Iran

Mehrzad Mehrbani |
Neuroscience Research Center, Institute of Neuropharmacology, Kerman University of Medical Sciences, Kerman, Iran

نشانی اینترنتی https://jkmu.kmu.ac.ir/article_92502_763b06de945d50fd0c0cd8b5a06f50d3.pdf
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