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Iranian Biomedical Journal، جلد ۲۶، شماره ۴، صفحات ۳۱۳-۳۲۳
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عنوان انگلیسی Strain-Specific Behavior of Mycobacterium tuberculosis in Interruption of Autophagy Pathway in Human Alveolar Type II Epithelial A549 Cells
چکیده انگلیسی مقاله Background: Autophagy induction has been shown to differ in magnitude depending on the mycobacterial species. However, few studies have investigated the specific autophagic capacity of different Mycobacterium tuberculosis (Mtb) strains in alveolar epithelial cells (ATs). This study aimed to elucidate the host autophagic response to different Mtb strains in ATs responsible for TB in the capital of Iran, Tehran.  
Methods: A549 cells were infected with three different Mtb clinical isolates (Beijing, NEW1, and CAS1/Delhi) and the reference strain H37Rv. Following RNA extraction, the expression of eight ATG genes, four mycobacterial genes, and three miRNAs was evaluated using quantitative RT-PCR.  
Results: The results revealed that all four strains influenced the autophagy pathway in various ways at different magnitudes. The Beijing and H37Rv strains could inhibit autophagosome formation, whereas the CAS and NEW1 strains induced autophagosome formation. The expression of genes involved in the fusion of autophagosomes to lysosomes (LAMP1) indicated that all the studied strains impaired the autophagolysosomal fusion; this result is not unexpected as Mtb can block the autophagolysomal fusion. In addition, the Beijing and H37RV strains prevented the formation of autophagic vacuoles, besides mycobacterial targeting of lysosomes and protease activity.
Conclusion: This preliminary study improved our understanding of how Mtb manages to overcome the host immune system, such as autophagy, and evaluated the genes used by specific strains during this process. Further studies with a large number of Mtb strains, encompassing the other main Mtb lineages, are inevitable.
کلیدواژه‌های انگلیسی مقاله A549 cells, Autophagy, MicroRNA, Mycobacterium tuberculosis
نویسندگان مقاله | Nasim Ebrahimifard
Pasteur Institute of Iran


| Shima Hadifar
Pasteur Institute of Iran


| Mansour Kargarpour Kamakoli
Pasteur Institute of Iran


| Ava Behrouzi
Pasteur Institute of Iran


| Sharareh Khanipour
Pasteur Institute of Iran


| Abolfazl Fateh
Pasteur Institute of Iran


| Seyed Davar Siadat
Pasteur Institute of Iran


| Farzam Vaziri
Pasteur Institute of Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-4741-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کامل
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