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Iranian Biomedical Journal، جلد ۲۷، شماره ۴، صفحات ۲۰۵-۲۱۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Anticancer Effect of Enterococcus faecium, Isolated from Vaginal Fluid, on Ovarian Cancer Cells |
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| چکیده انگلیسی مقاله |
Background: Given the association between cervicovaginal microbiota and OVC, we investigated the effect of Enterococcus faecium conditioned medium (CM) on OVC (Caov-4) cells.
Methods: CM was obtained from the bacterium E. faecium isolated from the vagina of healthy women. The Caov-4 cells were treated with varying concentrations of CM that comprised co-cultured bacteria with 0.2, 0.5, 1, 1.5, and 2 OD for 12, 24, and 48 h. The apoptosis and growth of cancer cells were evaluated by 4′,6-diamidino-2-phenylindole (DAPI) staining, flow cytometry, and DNA laddering assay. Moreover, the expression of PTEN, BAX, BCL2, and AKT1 genes were analyzed using real-time PCR.
Results: The CM at a concentration of 0.5 OD from the cultured bacteria and incubation time of 48 h showed the highest negative effect on the viability of cancer cells. The CM treatment increased DNA fragmentation and also induced apoptosis in Caov-4 cells. Interestingly, CM could decrease the expression of proapoptotic genes were less, while antiapoptotic genes were more than fluorouracil in the presence of CM.
Conclusion: CM of human-derived E. faecium could have an anticancer effect on OVC cells in a concentration- and time-dependent manner. This study demonstrated that E. faecium secretes anticancer substances into the CM, which could directly affect the viability and apoptosis of cancer cells. |
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| کلیدواژه‌های انگلیسی مقاله |
Apoptosis, Enterococcus faecium, Ovarian neoplasms |
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| نویسندگان مقاله |
| Soraya Pourmollaei
Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
| Azizeh Farshbaf-Khalili
Aging Research Institute, Physical Medicine, and Rehabilitation Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
| Abolfazl Barzegari
Research Center of Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
| Sepideh Bastani
Research Center of Pharmaceutical Nanotechnology, Biomedicine Institute, Tabriz University of Medical Sciences, Tabriz, Iran
| Soraya Babaie
Aging Research Institute, Physical Medicine, and Rehabilitation Research Centre, Tabriz University of Medical Sciences, Tabriz, Iran
| Amir Fattahi*
Womenʼs Reproductive Health Research Center, Tabriz University of Medical Sciences, Tabriz, Iran
| Mahnaz Shahnazi
Department of Midwifery, Faculty of Nursing and Midwifery, Tabriz University of Medical Sciences, Tabriz, Iran
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| نشانی اینترنتی |
http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5172-1&slc_lang=en&sid=1 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
Molecular Microbiology |
| نوع مقاله منتشر شده |
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