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Iranian Biomedical Journal، جلد ۲۷، شماره ۶، صفحات ۳۸۸-۳۹۶
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چکیده فارسی مقاله
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عنوان انگلیسی Decoding the Genetic and Structural Features of HPV16 E5 Oncogene in Cervical Cancer Isolates from Pakistan: A Pilot Study
چکیده انگلیسی مقاله Background: Many anogenital cancers are caused by high-risk human papillomavirus (HPV). The most common subtype is HPV16, which is prevalent in the world, including Pakistan. Various amino acid residues in HPV16 E5 are associated with high cell cycle progression and proliferation. Lack of studies on HPV16E5 in Pakistan prompted the current study. This is the first report on the occurrence of pathogenic E5 variant of HPV16 in tissue sections obtained from invasive cervical cancerous patients in Pakistan.
Methods: A subset of 11 samples from HPV-positive biopsies were subjected to E5 gene amplification using PCR and analyzed using bioinformatics programs. The bioinformatics analysis detected mutations causing structural variations, which potentially contribute to the oncogenic properties of proteins.
Results: The two-point mutations, C3979A and G4042A, observed in isolate 11 caused the substitution of isoleucine for leucine and valine at positions 44 and 65 in E5 protein. The rest of the isolates had Leu44Val65 amino acids. Intratypic variations and phylogenetic analysis revealed that the majority of the isolates were closely clustered with European-Asian lineage. Moreover, C3979A and G4042A contributed to higher degree of interactions with host receptors, i.e. epidermal growth factor receptor (EGFR).
Conclusion: This is the first study reporting HPV16 variants in a Pakistani population based on variations in the E5 region. Our findings indicate that isolate 11 has a strong interaction with the intracellular domain of EGFR, which may enhance the generation of downstream signals. Since this was a pilot study to explore E5 gene mutation, future studies with large samples are absolutely needed.
کلیدواژه‌های انگلیسی مقاله Human papillomavirus 16, Oncogenes, Uterine cervical neoplasms
نویسندگان مقاله | Naureen Ehsan Ilahi
Atta-Ur-Rehman School of Applied Biosciences, National University of Science and Technology, H-12 Campus, Hucknall Road, Islamabad, Pakistan


| Nayyer Siddiquec
Department of Molecular Biology and Genetics, Khyber Medical University, Peshawar, Pakistan


| Muhammad Ibrahim Rashid
Department of Biotechnology, Virtual University of Pakistan, Rawalpindi, Pakistan


| Mamoona Noreen
Department of Zoology, The Women University Multan, Multan, Pakistan


| Sheeba Murad
Atta-Ur-Rehman School of Applied Biosciences, National University of Science and Technology, H-12 Campus, Hucknall Road, Islamabad, Pakistan
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5260-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Microbiology
نوع مقاله منتشر شده مقاله کوتاه
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