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Iranian Biomedical Journal، جلد ۲۷، شماره ۶، صفحات ۳۴۹-۳۵۶
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عنوان انگلیسی Nona-Arginine Mediated Anti-E6 ShRNA Delivery Suppresses the Growth of Hela Cells in vitro
چکیده انگلیسی مقاله Background: The E6 oncoprotein of HPV plays a crucial role in promoting cell proliferation and inhibiting apoptosis, leading to tumor growth. Non-viral vectors such as nona-arginine (R9) peptides have shown to be potential as carriers for therapeutic molecules. This study aimed to investigate the efficacy of nona-arginine in delivering E6 shRNA and suppressing the E6 gene of HeLa cells in vitro.
Methods: HeLa cells carrying E6 gene were treated with a complex of nona-arginine and E6 shRNA. The complex was evaluated using gel retardation assay and FESEM microscopy. The optimal N/P ratio for R9 peptide to transfect HeLa cells with luciferase gene was determined.  Relative real-time PCR was used to evaluate the efficiency of mRNA suppression efficiency for E6 shRNA, while the effect of E6 shRNA on cell viability was measured using an MTT assay.
Results: The results indicated that R9 efficiently binds to shRNA and effectively transfects E6 shRNA complexes at N/P ratios greater than 30. Transfection with R9 and polyethylenimine complexes resulted in a significant toxicity compared to the scrambled plasmid, indicating selective toxicity for HeLa cells. Real-time PCR confirmed the reduction of E6 mRNA expression levels in the cells transfected with anti-E6 shRNA.
Conclusion: The study suggests that R9 is a promising non-viral gene carrier for transfecting E6 shRNA in vitro, with significant transfection efficiency and minimal toxicity.
کلیدواژه‌های انگلیسی مقاله Cell-penetrating peptides, E6 oncogene, Human papillomavirus 18, RNA interference
نویسندگان مقاله | Razieh Taghizadeh Pirposhteh
Department of Molecular Virology, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Ehsan Arefian
Department of Microbiology, School of Biology, College of Science, University of Tehran 1417614411, Iran


| Arash Arashkia
Department of Molecular Virology, Pasteur Institute of Iran, Tehran 1316943551, Iran


| Nasir Mohajel
Department of Molecular Virology, Pasteur Institute of Iran, Tehran 1316943551, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5467-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Pharmaceutical Biotechnology
نوع مقاله منتشر شده مقاله کامل
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