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Iranian Biomedical Journal، جلد ۲۸، شماره ۲، صفحات ۱۳۲-۱۳۹
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چکیده فارسی مقاله
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عنوان انگلیسی Polyethylene Glycol-Mediated Exosome Isolation: A Method for Exosomal RNA Analysis
چکیده انگلیسی مقاله
Background: Exosomal RNAs (ExoRNAs) offer valuable insights into their cellular origin. ExoRNA studies were faced with challenges in obtaining sufficient amounts of high-quality RNA. Herein, we aimed to compare three traditional exosome isolation methods to introduce an appropriate strategy to extract RNA from cancer-derived exosomes for further RNA analysis.
Methods: Exosomes were isolated through ultracentrifugation, precipitation kit, and size exclusion column chromatography, and then characterized by dynamic light scattering and transmission electron microscopy, followed by extracting total RNA. The quality and quantity of the extracted RNAs were assessed by a NanoDrop and 2.5% agarose gel electrophoresis.
Results: Extracted exosomes displayed a similar range of size and morphology. We found that polyethylene glycol-precipitation method resulted in a higher RNA yield with a 260/280 ratio of 1.9. The obtained exoRNA appeared as a smear in the agarose gel, indicative of small exoRNAs.
Conclusion: We provide researchers a suitable approach to isolate exosomes based on yield and purity of exoRNA.
 
کلیدواژه‌های انگلیسی مقاله Exosomes, Extracellular vesicles, MicroRNA
نویسندگان مقاله | Abdulwahab Teflischi Gharavi
Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran


| Azadeh Niknejad
Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran


| Saeed Irian
Department of Cell and Molecular Sciences, Faculty of Biological Sciences, Kharazmi University, Tehran, Iran


| Amirabbas Rahimi
Molecular Medicine Department, Biotechnology Research Center, Pasteur Institute of Iran, Tehran, Iran


| Mona Salimi
Department of Physiology and Pharmacology, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-5697-1&slc_lang=en&sid=1
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کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده Molecular Genetics & Genomics
نوع مقاله منتشر شده مقاله کوتاه
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