| چکیده انگلیسی مقاله |
Introduction: Polycystic ovary syndrome (PCOS) is a common and complex disease in women. Although the role of genetic factors in PCOS is strongly supported, the genes that are involved in the etiology of this syndrome have not yet been fully investigated. The aim of this study was the investigation of codons 164 and 27 of beta 2-adrenergic receptor gene polymorphism in women with PCOS. Methods: This cross-sectional study was performed in 2014 on 100 women with PCOS in Imam Khomeini Hospital, Tehran, Iran. 3 ml blood samples were collected for extraction of genomic DNA by the Qiagen DNA extraction kit, then gene bank based primers were prepared for PCR. Approved DNA samples were sequenced by Macrogen (row or sequence); resulting sequences were evaluated by the Chromas (ver. 2.4) application. The data were analyzed using the bootstrap method and SPSS software (ver.18). P < 0.05 was considered significant. Findings: The analysis of polymorphism in codon 164 in PCO women showed 44.4% statistically significant polymorphism (P< 0.002). Codon 27 did not show any significant polymorphism differences between PCO and non-PCO groups (P=0.606). Homozygous and heterozygous in both codons were significantly different (P=0.004 and P=0.0450, respectively). Conclusion: Inspection of beta2 adrenoceptor gene polymorphism in codons 164 and 27 showed that codon 164 is associated with PCO. |
| نویسندگان مقاله |
فریده ظفری زنگنه | farideh zangeneh
associate professor, vali-e-asr reproductive health research center, vali-e-asr hospital, imam khomeini hospital complex, tehran university of medical sciences, tehran, iran
دانشیار مرکز تحقیقات بهداشت باروری ولیعصر عج ، بیمارستان ولیعصر عج ، مجتمع بیمارستانی امام خمینی، دانشگاه علوم پزشکی تهران، تهران، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی تهران (Tehran university of medical sciences)
محمد مهدی نقی زاده | mohamad mahdi naghizadeh
instructor, department of community medicine, school of medicine, fasa university of medical sciences, fasa, iran
مربی گروه پزشکی اجتماعی، دانشکده پزشکی، دانشگاه علوم پزشکی فسا، فسا، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی فسا (Fasa university of medical sciences)
طیبه جعفریان | tayebeh jafarian
m.sc. student, department of biology, young researchers club, school of pharmaceutical sciences, tehran islamic azad university, tehran, iran
دانشجوی کارشناسی ارشد گروه بیولوژی و کلوپ محققان جوان، دانشکده علوم دارویی، دانشگاه آزاد اسلامی تهران، تهران، ایران
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
سازمان های دیگر: دانشگاه آزاد اسلامی تهران
سعیده نژاد فتح مقدم | saeedeh nejad fateh moghadam
m.sc. student, department of biology, young researchers club, school of pharmaceutical sciences, tehran islamic azad university, tehran, iran
دانشجوی کارشناسی ارشد گروه بیولوژی و کلوپ محققان جوان، دانشکده علوم دارویی، دانشگاه آزاد اسلامی تهران، تهران، ایران
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
سازمان های دیگر: دانشگاه آزاد اسلامی تهران
آرش سلمانی | arash salmani
ph.d. student of genetics, department of genetics, school of medicine, mashhad university of medical sciences, mashhad, iran
دانشجوی دکترای ژنتیک، گروه ژنتیک، دانشکده پزشکی، دانشگاه علوم پزشکی مشهد، مشهد، ایران
سازمان اصلی تایید شده: دانشگاه علوم پزشکی مشهد (Mashhad university of medical sciences)
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