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Medical Laboratory Journal، جلد ۱۰، شماره ۵، صفحات ۶۵-۷۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Prevalence of blaoxa-1 and blashv Genes in E. coli Isolates from Hospitalized Patients in Rasht |
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| چکیده انگلیسی مقاله |
ABSTRACT Background and Objective: Extended-spectrum beta-lactamases (ESBL) are widely produced by Escherichia coli strains. The aim of this study was to determine frequency of blaOXA-1 and blaSHV genes in E.coli strains isolated from patients hospitalized in city of Rasht, Iran. Methods: In this cross-sectional study, 200 samples were collected from patients. The E. coli strains were identified using morphological characteristics and biochemical tests. Antimicrobial susceptibility testing was performed. The prevalence of the blaOXA-1 and blaSHV genes in the E. coli isolated was assessed by PCR method. Results: Overall, 160 E. coli strains were isolated. Of these, 83 (51.9%) showed ESBL activity while 71 (48.1%) did not. All positive strains were resistant to cephalothin. Moreover, 98.8% of ESBL-producing strains were resistance to amoxicillin, cefotaxime and ceftriaxone. The prevalence of the blaOXA-1 and blaSHV genes in ESBL-producing strains were 45% and 17%, respectively. In addition, 28.9% of the strains had both genes while the genes were absent in 9.6% of the strains. Conclusion: Prevalence of the blaOXA-1gene is higher than that of the blaSHV gene. The absence of both genes in some isolates indicates the possible role of other genes in the ESBL activity. Keywords: Prevalence, ESBLs, Escherichia coli, blaOXA-1gene, blaSHV gene. |
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| نویسندگان مقاله |
محمد فایزی قاسمی | mohammad faezi ghasemi
department of microbiology, lahijan branch, islamic azad university, lahijan iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
سیده نگین dibadji | seyede negin dibadji
department of microbiology, lahijan branch, islamic azad university, lahijan iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://goums.ac.ir/mljgoums/browse.php?a_code=A-10-1-438&slc_lang=en&sid=fa |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/168/article-168-326933.pdf |
| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
تخصصی |
| نوع مقاله منتشر شده |
تحقیقی |
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