| چکیده انگلیسی مقاله |
Background: Phenobarbital is a sedative drug that belongs to the barbiturate class. Barbiturates work by reducing the excitability of neurons. The present study aimed to evaluate the effects of phenobarbital on the embryos of adult female BALB/c mice.
Materials and Methods: In this study, 60 adult female BALB/c mice weighing approximately 24 to 26 g were classified into six groups: control, sham, and treatment groups. Phenobarbital ampoules were used for the study, and the lethal dose (LD50) in vivo was calculated to be 3.84 ml/kg.bw. Intraperitoneal injections were administered on days 3, 4, 5, and 6 of pregnancy. On day 7 of pregnancy (following the last injection), the mice were euthanized, weighed, and dissected. Their uterus and ovaries were rinsed with sterile physiological serum and evaluated both microscopically and macroscopically. The results obtained were analyzed using SPSS software and subjected to an ANOVA test.
Results: Based on the results of the study, phenobarbital caused syndactyly (gluing of fingers and toes), exohepatic, exencephaly, spinabifida, lack of motor organ formation, asymmetry in motor organ orientation, liver enlargement, weight loss, CR size reduction, subcutaneous hemorrhages, C-shaped body, and umbilical hernia. Additionally, a significant decrease (P<0.05) was reported in fetal weight, placental weight, placental diameter, and CR.
Conclusion: The current study indicated that phenobarbital likely has a negative impact on embryos during the embryonic development stage, leading to miscarriage, abnormalities, and infertility. Therefore, serious precautions should be taken to avoid the use of this drug during pregnancy.
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| نویسندگان مقاله |
منیره منفردی | Monireh Monfaredi
Animal-Cellular and Developmental Biology, Cellular and Molecular Research Center, Iran University of Medical Sciences, Tehran, Iran
بیولوژی ﺟﺎﻧﻮری-ﺳﻠﻮلی و ﺗﻜﻮینی، مرکز تحقیقات سلولی و مولکولی، دانشگاه علوم پزشکی ایران، تهران، ایران
محسن قیاسی | Mohsen Ghiasi
Cellular and Molecular Biology, Rajaie Cardiovascular Medical and Research Center, Iran University of Medical Sciences, Tehran, Iran
بیولوژی ﺳﻠﻮلی و مولکولی، ﻣﺮﻛﺰ ﺗﺤﻘﯿﻘﺎت ﻗﻠﺐ و ﻋﺮوق شهید رﺟﺎیﻲ، مرکز آموزشی، تحقیقاتی و درمانی قلب و عروق شهید رجایی، دانشگاه علوم پزشکی ایران، تهران، ایران
منیره موحدی | Monireh Movahedi
Department of Biochemistry, Faculty of Biological Sciences, North Tehran Branch, Islamic Azad University, Tehran, Iran
گروه بیوشیمی، دانشکده‌ی علوم زیستی، دانشگاه آزاد اسلامی، واحد تهران شمال، تهران، ایران
پروین تراب زاده | Parvin Torabzadeh
Department of Biology, Karaj Branch, Islamic Azad University, Karaj, Iran
گروه زیست شناسی، دانشگاه آزاد اسلامی، واحد کرج، کرج، ایران
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