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Veterinary Research Forum، جلد ۱۵، شماره ۶، صفحات ۳۰۹-۳۱۶
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عنوان انگلیسی Identification of a lytic bacteriophage against clinical isolates of Salmonella typhimurium in turkey poults
چکیده انگلیسی مقاله The poultry products are known as a source of zoonotic and multi-drug resistant pathogens, especially Salmonella spp. The objective of this study was using bacteriophages as an alternative anti-microbial agent against Salmonella typhimurium isolate from turkey poults. The antibiotic susceptibility test was used to identify the antibiotic resistance pattern of the isolates. The bacteriophage was purified, enhanced and titrated using the Spot test and double layer agar (DLA) techniques after being isolated from a chicken slaughterhouse and sewage treatment facility. By determining the morphological characteristics of resulting plaque, the specificity and host range of the phage were studied on S. typhimurium isolates. A total number of 22 suspected Salmonella isolates were confirmed biochemically positive in sample by cultures method. Nine of these isolates (40.90%) were identified as S. typhimurium by polymerase chain reaction. All of isolates (100%) were resistant to chloramphenicol, doxycycline, kanamycin, florfenicol, rifampin, and erythromycin. Seven isolates (77.77%) were resistant to amoxicillin and nalidixic acid. The plaques were present with 3.00 ± 0.22 mm in diameter on the culture of 6 out of 9 (66.66%) isolates of S. typhimurium on brain heart infusion broth using DLA method. The amount of phage titer was 7.60 × 107 phage forming unit mL-1 and its multiplicity of infection value was calculated as 5.06 × 10-2 based on obtained results. In place of antibiotics, the multi-drug resistant (MDR) S. typhimurium was successfully destroyed by the isolated bacteriophage from wastewater. In vitro settings were used in this investigation to identify the efficient bacteriophages against MDR S. typhimurium.
کلیدواژه‌های انگلیسی مقاله Antibiotics,Bacteriophage,Salmonella typhimurium,Turkey poults
نویسندگان مقاله Masoud Amini |
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Abdulghaffar Ownagh |
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Amir Tokmachi |
Department of Microbiology, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

Manochehr Allymehr |
Department of Poultry Health and Diseases, Faculty of Veterinary Medicine, Urmia University, Urmia, Iran

نشانی اینترنتی https://vrf.iranjournals.ir/article_709152_dc5945f4c634abcfa645e0bf95e686e4.pdf
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