این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Biomedical Journal، جلد ۲۱، شماره ۲، صفحات ۸۴-۹۳
عنوان فارسی Identification and Evaluation of Novel Drug Targets against the Human Fungal Pathogen Aspergillus fumigatus with Elaboration on the Possible Role of RNA-Binding Protein
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Identification and Evaluation of Novel Drug Targets against the Human Fungal Pathogen Aspergillus fumigatus with Elaboration on the Possible Role of RNA-Binding Protein
چکیده انگلیسی مقاله Bakground: Aspergillus fumigatus is an airborne opportunistic fungal pathogen that can cause fatal infections in immunocompromised patients. Although the current anti-fungal therapies are relatively efficient, some issues such as drug toxicity, drug interactions, and the emergence of drug-resistant fungi have promoted the intense research toward finding the novel drug targets. Methods: In search of new antifungal drug targets, we have used a bioinformatics approach to identify novel drug targets. We compared the whole proteome of this organism with yeast Saccharomyces cerevisiae to come up with 153 specific proteins. Further screening of these proteins revealed 50 potential molecular targets in A. fumigatus. Amongst them, RNA-binding protein (RBP) was selected for further examination. The aspergillus fumigatus RBP (AfuRBP), as a peptidylprolyl isomerase, was evaluated by homology modeling and bioinformatics tools. RBP-deficient mutant strains of A. fumigatus were generated and characterized. Furthermore, the susceptibility of these strains to known peptidylprolyl isomerase inhibitors was assessed. Results: AfuRBP-deficient mutants demonstrated a normal growth phenotype. MIC assay results using inhibitors of peptidylprolyl isomerase confirmed a higher sensitivity of these mutants compared to the wild type. Conclusion: Our bioinformatics approach revealed a number of fungal-specific proteins that may be considered as new targets for drug discovery purposes. Peptidylprolyl isomerase, as a possible drug target, was evaluated against two potential inhibitors and the promising results were investigated mechanistically. Future studies would confirm the impact of such target on the antifungal discovery investigations.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله سعید ملک زاده | saeid malekzadeh
bioinformatics and drug design unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سروش سرداری | soroush sardari
bioinformatics and drug design unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

پریسا azerang | parisa azerang
bioinformatics and drug design unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

درسا خراسانی زاده | dorsa khorasanizadeh
fungal biotechnology unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

سولماز آقا امیری | solmaz agha amiri
fungal biotechnology unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

محمد عزیزی | mohammad azizi
fungal biotechnology unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نازنین مهاجرانی | nazanin mohajerani
fungal biotechnology unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

وحید خلج | vahid khalaj
fungal biotechnology unit, medical biotechnology department, biotechnology research center, pasteur institute of iran, tehran 1316943551, iran

سازمان اصلی تایید شده: انستیتو پاستور ایران (Pasteur institute of iran)

نشانی اینترنتی http://ibj.pasteur.ac.ir/browse.php?a_code=A-10-1-642&slc_lang=en&sid=en
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/125/article-125-316093.pdf
کد مقاله (doi)
زبان مقاله منتشر شده fa
موضوعات مقاله منتشر شده Medical Biotechnology
نوع مقاله منتشر شده مقاله کامل
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات