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Iranian Journal of Basic Medical Sciences، جلد ۲۷، شماره ۸، صفحات ۹۸۵-۹۹۵
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Fucosyltransferase 3 and 8 promote the metastatic capacity of cancer stem-like cells via CD15s and E-cadherin in esophageal cancer |
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| چکیده انگلیسی مقاله |
Objective(s): Esophageal cancer stem cells (ECSCs) have been identified as the subset of cells within esophageal squamous cell carcinoma that possess tumorigenic, invasive, and metastatic properties. One important aspect of cancer metastasis is the binding of sialyl-Lewis X (CD15s) with E- or P-selectin, which facilitates the adhesion and migration of cancer cells to distant sites. This study was conducted to investigate the impact of fucosylation processes on the metastatic behavior of ECSCs.Materials and Methods: The esophageal cancer cell line (KYSE-30) was cultured and divided into control and 2F-peracetyl fucose (2F-PerAcFuc) treated groups. Spheres were harvested from these cultures. Cell invasion assay and qPCR were conducted to examine migration and marker expression in both groups. Cancer cell line-derived xenografts were established in nude mice to validate findings in vivo.Results: Our results initially indicated that the addition of 2F-PerAcFuc, an inhibitor of fucosylation, resulted in the down-regulation of the Fut3/CD15s pathway in both cancer stem-like cells and the xenograft model. Measurements of subcutaneous xenograft tumor volume revealed a significant decrease in tumor size among nude mice after treatment with 2F-PerAcFuc. Additionally, a reduction in Fut8/E-cadherin levels was observed in the xenograft model of nude mice. Furthermore, the administration of 2F-PerAcFuc lowered the levels of fucosylated glycoconjugates in nude mice.Conclusion: Our data suggest that inhibition of fucosyltransferase 3 and 8 can reduce the metastatic capacity of cancer stem-like cells by down-regulating CD15s and E-cadherin in a mouse model of esophageal cancer. |
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| کلیدواژه‌های انگلیسی مقاله |
E-cadherin, Esophageal cancer stem - cells, 2F-PerAcFuc, Fucosyltransferase 3 and 8, Sialyl lewis X |
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| نویسندگان مقاله |
| Aliakbar Rostami Abookheili
Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran|Shefa Neuroscience Research Center, Khatam Al-anbia Hospital, Tehran, Iran
| Jahanbakhsh Asadi
Metabolic Disorders Research Center, Golestan University of Medical Sciences, Gorgan, Iran|Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
| Ayyoob Khosravi
Department of Molecular Medicine, Faculty of Advanced Medical Technologies, Golestan University of Medical Sciences, Gorgan, Iran|Stem Cell Research Center, Golestan University of Medical Sciences, Gorgan, Iran
| Ali Gorji
Shefa Neuroscience Research Center, Khatam Al-anbia Hospital, Tehran, Iran|Epilepsy Research Center, Westfälische Wilhelms-Universität Münster, Germany
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| نشانی اینترنتی |
https://ijbms.mums.ac.ir/article_24174.html |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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