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Nanomedicine Journal، جلد ۴، شماره ۱، صفحات ۸-۱۶
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عنوان انگلیسی Optimization of conditions for gene delivery system based on PEI
چکیده انگلیسی مقاله Objective(s): PEI based nanoparticle (NP) due to dual capabilities of proton sponge and DNA binding is known as powerful tool for nucleic acid delivery to cells. However, serious cytotoxicity and complicated conditions, which govern NPs properties and its interactions with cells practically, hindered achievement to high transfection efficiency. Here, we have tried to optimize the properties of PEI/ firefly luciferase plasmid complexes and cellular condition to improve transfection efficiency. Materials and Methods: For this purpose, firefly luciferase, as a robust gene reporter, was complexed with PEI to prepare NPs with different size and charge. The physicochemical properties of nanoparticles were evaluated using agarose gel retardation and dynamic light scattering. MCF7 and BT474 cells at different confluency were also transfected with prepared nanoparticles at various concentrations for short and long times. Results: The branched PEI can instantaneously bind to DNA and form cationic NPs. The results demonstrated the production of nanoparticles with size about 100-500 nm dependent on N/P ratio. Moreover, increase of nanoparticles concentration on the cell surface drastically improved the transfection rate, so at a concentration of 30 ng/ìl, the highest transfection efficiency was achieved. On the other side, at confluency between 40-60%, the maximum efficiency was obtained. The result demonstrated that N/P ratio of 12 could establish an optimized ratio between transfection efficiency and cytotoxicity of PEI/plasmid nanoparticles. The increase of NPs N/P ratio led to significant cytotoxicity. Conclusion: Obtained results verified the optimum conditions for PEI based gene delivery in different cell lines.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله رویا چراغی | roya cheraghi
department of nanobiotechnology, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

محسن علیپور | mohsen alipour
department of nanobiotechnology, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

محبوبه نظری | mahboobeh nazari
monoclonal antibody research center, avicenna research institute, acecr, tehran, iran


سامان حسین خانی | saman hosseinkhani
department of biochemistry, faculty of biological sciences, tarbiat modares university, tehran, iran

سازمان اصلی تایید شده: دانشگاه تربیت مدرس (Tarbiat modares university)

نشانی اینترنتی http://nmj.mums.ac.ir/article_8047.html
فایل مقاله اشکال در دسترسی به فایل - ./files/site1/rds_journals/295/article-295-313991.pdf
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زبان مقاله منتشر شده en
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نوع مقاله منتشر شده 1
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