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Iranian Journal of Microbiology، جلد ۱۶، شماره ۲، صفحات ۲۵۱-۲۵۷
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عنوان انگلیسی In vitro anti-HSV1 activity of aqueous extract of Areca catechu L.
چکیده انگلیسی مقاله Background and Objectives: HSV-1 is known as a very contagious virus and the main cause of cold sores or fever blisters. Herein, the aqueous extract of Areca catechu L. was evaluated for its anti-HSV-1 activity, compared to the standard control (acyclovir). Also, the effect of extract on the expression of UL46 and US6 genes that accumulate late in viral infection, was studied. Materials and Methods: The aqueous extract was obtained by the maceration of powdered plant in boiling water. Its antiviral activity was evaluated on Vero cells infected with HSV-1 at different times: 2 h pre-infection, simultaneous infection, and 4 h post-infection, using MTT assay. The effect of extract on the expression of genes was investigated with quantitative real-time PCR. Results: The aqueous extract of A. catechu induced the inhibition of infection with the IC50 value of 110.52 ± 1.36 μg/ml. Also, it reduced the expression of UL46 when it was added 2 h pre-infection at 100 μg/ml. Moreover, reduction of expression of US6 was observed at the same concentration when the extract was used simultaneously with the occurrence of infection and 4 h post-infection. Conclusion: A. catechu can be considered an essential element of natural-based anti-HSV-1 agents.
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نویسندگان مقاله | Mahsa Zamanian
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Emad Asgari
Department of Theriogenology, School of Veterinary Medicine, University of Tehran, Tehran, Iran


| Sahar Afaridoon
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Tahmineh Akbarzadeh
Department of Medicinal Chemistry, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran; Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Zohreh Sharifi
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Mina Saeedi
Persian Medicine and Pharmacy Research Center, Tehran University of Medical Sciences, Tehran, Iran; Medicinal Plants Research Center, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4335
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