| چکیده انگلیسی مقاله |
Background and purpose : Cholestasis is caused by a malfunction of the biliary liver system. Oxidative stress plays an essential role in the progression of cholestasis. This study aimed to investigate the antioxidant and hepatoprotective effects of ethanolic extract of Juniperus excelsa M. Bieb (JE) fruits on hepatic impairment induced by bile duct ligation (BDL) in rats. Experimental approach: Forty male Wistar rats were randomly divided into 4 groups; sham control + vehicle (SC), BDL + vehicle (BDL), BDL + JE extract (BDL + JE), and SC + extract (SC + JE). One day after surgery, the animals were treated with vehicle or ethanolic extract of JE (500 mg/kg/day) for 7 days. Finally, the blood was taken for biochemical and oxidative stress analysis. Furthermore, the liver tissue of rats was removed for histological examination. Findings/Results : Treatment with the extract of JE decreased the ALP level, whereas it enhanced total protein content compared to the BDL group. Also, JE increased the activity of SOD and GPx, as well as FRAP content compared to the BDL group; while it did not significantly affect the levels of MDA and inflammation markers. However, JE could not improve BDL-induced histopathological alterations in hepatic tissue. Conclusion and implication : This study demonstrated that JE may be useful as an adjuvant therapy by attenuating ALP activity, increasing serum total protein and FRAP content, as well as improving the antioxidant enzymes activity of SOD and GPx. However, further research is warranted to explore the other underlying mechanisms of action. |
| کلیدواژههای انگلیسی مقاله |
Anti-inflammatory,Antioxidant,Bile duct ligation,Juniperus excelsa,Liver,Rat. |
| نویسندگان مقاله |
| Ghaidafeh Akbari
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
| Mohammad Reza Abasi
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
| Maral Gharaghani
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
| Sadegh Nouripoor
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
| Nasrin Shakerinasab
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
| Mahdokht Azizi
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
| Marjan Salahi
Department of Physiology, Behbahan Faculty of Medical Sciences, Behbahan, Iran.
| Farzaneh Karimi
Department of Pharmacognosy and Pharmaceutical Biotechnology, Faculty of Pharmacy, Kermanshah University of Medical Sciences, Kermanshah, Iran.
| Mahdieh Eftekhari
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran.
| Damoun Razmjoue
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran.
Department of Clinical Biochemistry, Faculty of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran.
| Amir Hossein Doustimotlagh
|