این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Research in Pharmaceutical Sciences، جلد ۱۹، شماره ۲، صفحات ۱۷۸-۱۸۷
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی In vitro and in vivo assessment of indomethacin-induced genotoxicity: protection by curcumin
چکیده انگلیسی مقاله Background and purpose: Indomethacin is one of the most widely used non-steroidal anti-inflammatory drugs. This study aimed to investigate the protective effects of curcumin against indomethacin-induced genotoxicity. Experimental approach: For in vitro studies, human peripheral blood lymphocytes were obtained from a healthy volunteer and treated for 24 h as follows: vehicle control, indomethacin at 100 and 200 μM, indomethacin (100 μM and 200 μM) plus curcumin (27 μM). For in vivo experiments, mice received a single i.p dose of curcumin (100 mg/kg) and after 30 min genotoxicity induction was carried out by a single                             i.p injection of indomethacin at 10, 20, and 40 mg/kg. After 24 h, bone marrow cells were obtained from mice femurs. Genotoxicity was evaluated using a micronucleus assay. Oxidative damage was also inspected both                   in vitro and in vivo . Findings/Results: In-vitro studies indicated that co-treatment with curcumin caused a significant decrease in the average micronuclei percentage and MDA level, and a significant increase in GSH concentration compared to the groups treated only with indomethacin. In-vivo findings revealed that pretreatment with curcumin induced a significant increase in the average ratio of polychromatic erythrocyte/normochromic erythrocyte, GSH concentration and caused a significant decrease in the average percentage of micronuclei and MDA level, in comparison with the group treated only with indomethacin. Conclusion and implications: Curcumin attenuated indomethacin-induced genotoxicity both in vitro and                  in vivo . These effects might be partially exerted by decreasing oxidative stress. Further studies are required to elucidate the exact genoprotective mechanism of curcumin against indomethacin.    
کلیدواژه‌های انگلیسی مقاله Curcumin,Genotoxicity,Indomethacin,Oxidative stress.
نویسندگان مقاله | Ehsan Zamani
Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Student Research Committee, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.


| Reza Alipour Klour
Student Research Committee, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran. Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences,Rasht, Iran.


| Amirreza Gholami Shekarsarayi
Department of Pharmacology and Toxicology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.


| Foad Ghazizadeh
Department of Pharmaceutical Biotechnology, School of Pharmacy, Guilan University of Medical Sciences, Rasht, Iran.


| Mehdi Evazalipour


نشانی اینترنتی http://rps.mui.ac.ir/index.php/jrps/article/view/2241
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده Original Article
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات