| چکیده انگلیسی مقاله |
Statement of the Problem: Periodontitis is an inflammatory disease that causes bone loss. Some patients do not respond well to the classic treatment and need therapies that minimize bone loss, the main sequel of the disease. Chenopodium ambrosioides L. has stood out due to its anti-inflammatory and anti-oxidative activities. However, no study has yet investigated its effect on periodontitis.
Purpose: This study aimed to evaluate the bone protective effect of Chenopodium ambrosioides L. (CAL) extract on ligature-induced periodontitis model in rats.
Materials and Method: For this, a pre-clinical assay was performed, using male Wistar rats divided into 3 groups: Naive (N) (n=6), not submitted to any procedure; Saline (SAL) (n=6), submitted to ligature-induced periodontitis and receiving 2 ml/kg of 0.9% saline solution; and CAL extract, which was subdivided into 3 subgroups (n=6/subgroup) receiving the CAL at 3 (CAL3), 10 (CAL10) or 30 mg/kg (CAL30). All agents were given, by oral gavage, 30 min before periodontitis induction and daily until euthanasia (11th day). By then, maxillae were removed for macroscopic, histological, and histometric analyses. Kidneys, liver, and stomach were collected to evaluate the safety of CAL extract. High-performance liquid chromatography (HPLC) assay was used to investigate the flavonoid content in the extract.
Results: Chenopodium ambrosioides L. extract at 30mg/kg showed a reduction by 58% in bone loss marked by an increase (+35%) in the number of osteoblasts and a reduction (-51%) on the number of osteoclasts (p< 0.05). No significant alteration in the liver, kidney, or stomach was seen. Rutin was the main flavonoid found.
Conclusion: In summary, it was observed that Chenopodium ambrosioides L. extract has shown important anti-inflammatory and bone anabolic and anti-resorptive properties without causing toxicity in the main organs. Rutin, as the main flavonoid of the extract, seems to be responsible for the beneficial effect of this agent. |
| نویسندگان مقاله |
Diego Thiers Oliveira Carneiro |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Michael Douglas Da Silva |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Karla Vanessa Pinto Vasconcelos |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Romero Dias |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Vanessa Costa |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Raquel Felipe Vasconcelos |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Bárbara Carneiro |
Post Graduation Program of Dentistry, School of Dentistry, Federal University of Ceará, Fortaleza, Brazil.
Gisele Barreto |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Mirna Marques |
Post Graduation Program in Health Science, School of Medicine, Federal University of Ceará, Sobral, Brazil.
Hellíada Chaves Vasconcelos |
Post Graduation Program in Health Science, School of Medicine, Federal University of Ceará, Sobral, Brazil.
Howard Lopes Ribeiro Júnior |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Jonas Nogueira Ferreira Maciel Gusmão |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Helson Da Silveira |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Renata Leitão |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Gerly Anne De Castro Brito |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Karuza Maria Alves Pereira |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Delane Viana Gondim |
Dept. of Morphology, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
Paula Goes |
Dept. of Pathology and Legal Medicine, School of Medicine, Federal University of Ceará, Fortaleza, Brazil.
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