این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Journal of Arthropod-Borne Diseases، جلد ۱۷، شماره ۳، صفحات ۱۶۵۰-۱۶۵۰
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Drosophila melanogaster Laboratory Rearing for Wolbachia-Based Control Programs, a Component of Dengue Control
چکیده انگلیسی مقاله Background: Drosophila melanogaster flies are smooth, low upkeep and safe model organisms, they can be effortless­ly used in different fields of life sciences like genomics, biotechnology, genetics, disease model, and Wolbachia-based approaches to fight vectors and the pathogens they transmit. Methods: Fruit fly specimens were collected in 25 districts (14 provinces) of Iran and their morphological recognition was proven by molecular analysis based on sequence homology of mitochondrial COI barcode region. Essential in­formation and specific requirements were provided for laboratory rearing of D. melanogaster. Results: Drosophila melanogaster colonies were found in 23 out of 25 districts. Also, five related species coincident with D. melanogaster were reported in this study including D. ananassae/D. parapallidosa, D. hydei, D. repleta, Zapri­onus indianus (Diptera: Drosophilidae), and Megaselia scalaris (Diptera: Phoridae). The Iranian D. melanogaster mo­lecular signature and their rearing techniques have been described here. The complete life cycle, from (egg to adult), takes approximately 8 days at 25 °C. Some biological points have been presented with highlighting capturing, rearing, culturing, and embryo collection along with primitive recognition and segregation between females and males have been presented. A recipe for culture media and the quantity of various ingredients have been provided. Conclusion: This is the first report on the D. repleta and D. ananassae/D. parapallidosa species for the country. Re­sults of this study provide efficient and effective rearing procedures which are requirement for both small-scale for fa­cilitating entomological research and large-scale use in justifiable vector control management such as disease model or Dengue control.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Nazanin Naseri-Karimi
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Hassan Vatandoost
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Sedaghat
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Seyed Hassan Moosa-Kazemi
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Fardin Amidi
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Mohammad Oshaghi
Department of Vector Biology and Control of Diseases, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
نشانی اینترنتی https://jad.tums.ac.ir/index.php/jad/article/view/1650
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات