این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Veterinary Medicine، جلد ۷، شماره ۱، صفحات ۱۵-۲۲
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The mRNAexpression study on small amount of Theileria annulata lymph node biopsy sample using SMART-cDNA technology
چکیده انگلیسی مقاله BACKGROUND: A major issue in many gene expression
studies utilizing small amount of biological materials is the
limited quantity of RNApurified from clinical samples, which is
often used for RT-PCR or standard Northern blot analysis.
OBJECTIVES: The SMART cDNA synthesis method and subsequent
SMART-cDNA-PCR technique was used to analyse 3
genes in macroschizonts of Theileria annulata in small lymph
node biopsy material. METHODS: The SMART-cDNA of TaSp
gene was cloned in pTZ57R/T-vector and sequenced. We focused
on genes encoding surface proteins TaSp, TaD and HSP70.
RESULTS: Our results showed that SMART cDNA dependably
reproduces the expression profile found in messenger RNA. The
RT-SMART-PCR showed the amplification of the processed
mRNAs. The sequencing analysis showed that the amplified
cDNA was coded for TaSp protein in Theileria annulata.
CONCLUSIONS: It was concluded that the SMART PCR
technique is practical for amplification of complete sequence of
mRNAs in the form of cDNAs, and therefore for gene expression
studies if only small amounts of starting material are available.
کلیدواژه‌های انگلیسی مقاله Theileria annulata, SMART-cDNA, TaSp, TaD, HSP70
نویسندگان مقاله Nastaran Sadr Shirazi |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran

Parviz Shayan |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran; Institute of Molecular Biological System Transfer (MBST), Tehran, Iran

Berigiteh Eckert |
Institute of Molecular Biological System Transfer (MBST), Tehran, Iran

Elaheh Ebrahimzadeh |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran

Sedigheh Jafari |
Department of Parasitology, Faculty of Veterinary Medicine, University of Tehran,Tehran, Iran

نشانی اینترنتی https://ijvm.ut.ac.ir/article_32019_3e488d7a07aad80a635656ada23e1571.pdf
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات