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Iranian Journal of Chemistry and Chemical Engineering، جلد ۳۹، شماره ۴، صفحات ۱۷۳-۱۸۱
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عنوان انگلیسی Determination of Fat-Soluble Vitamins A, D3, and E in Infant Formula and Milk Powder Using High-Performance Liquid Chromatography with Photodiode Array Detection: Jordan Market as a Case Study
چکیده انگلیسی مقاله A new, simple, rapid, and sensitive reversed-phase High-Performance Liquid Chromatography-Photodiode Array (HPLC-PDA) method was developed and validated for the simultaneous analysis of fat-soluble vitamins A, D3, and E. The method required a simple sample preparation step of saponification with aqueous KOH and extraction with n-hexane. The method was validated in terms of linearity, accuracy, precision, stability, detection limits, and recovery. The method has the advantage of simultaneous determination of vitamins A, D3, and E in a short run time of 10 min. The method was applied successfully for the determination of vitamins A, D3, and E in some infant formula and milk powder from the Jordanian market. Based on this study, vitamin Content in all brands was within 90.0-410% of the labeled value. Vitamin D3 content in the studied brands was within 100.0-850%, while vitamin E content in all brands was less than 48%. The results showed large variation and discrepancy of most vitamins’ contents, which were not in good agreement with the manufacturer label value, though they were below toxic levels.
کلیدواژه‌های انگلیسی مقاله Fat-soluble vitamins,Infant formula,Milk Powder,HPLC-PDA
نویسندگان مقاله Feras Alali |
Pharmaceutical Sciences College of Pharmacy, QU Health, Qatar University, QATAR

Mohammad Amayreh |
of Chemistry, Mut’ah University, Al-Karak 61710, JORDAN and Department of Chemistry, Faculty of Science, Al-Balqa Applied University, P.O.Box 19117, Al-Salt, JORDAN

Adnan Massadeh |
Department of Medicinal Chemistry and Pharmacognosy, Jordan University of Science and Technology, Irbid 22110, JORDAN

Abdullah El-Alali |
Department of Chemistry, Mut’ah University, Al-Karak 61710, JORDAN

نشانی اینترنتی https://ijcce.ac.ir/article_34728_3558f86dce70c5194df4e389e9fabf3b.pdf
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