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International Journal of Engineering، جلد ۳۲، شماره ۹، صفحات ۱۲۲۵-۱۲۳۰
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Hemp Fibres as Novel Green Support Material for Immobilization of <i>Acidithiobacillus ferrooxidans</i> |
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| چکیده انگلیسی مقاله |
The suitability of hemp fibres as green support material for immobilization of Acidithiobacillus ferrooxidans cells in ferrous iron bio-oxidation process was investigated. A series of experiments were carried out in shake flasks and bioreactor for both cell immobilization and iron bio-oxidation. In flask experiments, five successive batch colonization cycles resulted in 6-fold increase in ferrous iron bio-oxidation rate. Evaluation of immobilized cells activity through two approaches as wet and dry storage demonstrated the higher activity of wet stored bacteria than dry ones after a period of two months. The dry stored bacteria lost almost all their activities after eight weeks. Whereas, the activity of wet stored samples was 30% at the same time. In bioreactor experiments, use of a packed-bed bioreactor with hemp fibres, the maximum ferrous iron bio-oxidation rate of 2.46 kg m-3 h-1 was obtained at dilution rate of 3.97 h-1, and retention time of 0.25 h. Our experimental results could pave a new platform for the application of hemp fibre as an inexpensive and environmentally friendly support material in bacterial cell immobilization, especially in ferrous iron bio-oxidation process using acidophilic bacteria. |
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| کلیدواژه‌های انگلیسی مقاله |
Acidithiobacillus Ferrooxidans,
Bio-oxidation,
Hemp Fibres,
Immobilization,
Packed-bed bioreactor |
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| نویسندگان مقاله |
Neda Akhlaghi |
Biotechnology Research Laboratory, Faculty of Chemical Engineering, Noshirvani University of Technology, Babol, Iran
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| نشانی اینترنتی |
http://www.ije.ir/article_93861_15de88da4d8100c6f2afbdc4453f48a3.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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