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International Journal of Engineering، جلد ۳۳، شماره ۴، صفحات ۵۱۳-۵۱۹
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عنوان انگلیسی Immobilization of Laccase from <i>Trametes hirsuta</i> onto CMC Coated Magnetic Nanoparticles
چکیده انگلیسی مقاله In this study, Fe3O4/CMC magnetic nanoparticles were synthesized through co-precipitation method. Afterward, laccase from Trametes hirsuta was immobilized onto Carboxymethyl cellulose (CMC)-coated magnetic Fe3O4 nanoparticles by covalent bonding between carboxyl groups of carboxymethyl cellulose and amine group of laccases. Also, the resulted magnetic nanoparticles and immobilized laccase were characterized by Fourier-transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and dynamic light scattering (DLS) analysis. Moreover, the vital factors in enzyme immobilization, such as contact time, amount of N-hydroxysuccinimide (NHS), and the amount of nanoparticles were optimized, which successively 48 h, 0.01 g, and 0.0125 g were achieved for 0.01g of N-ethyl-N′-(3-dimethylaminopropyl)carbodiimide (EDC). Activity recovery of 51 ± 0.8% was achieved by optimizing the immobilization process. The results also indicated that the loading of laccase onto carboxymethyl cellulose-coated Fe3O4 nanoparticles was approximately 120 (mg/g). Finally, the immobilized laccases on magnetic support could save nearly 50% of their initial activity after five consecutive cycles.  
کلیدواژه‌های انگلیسی مقاله Laccase, Immobilization, Fe3O4/CMC, Trametes hirsuta
نویسندگان مقاله S. Sadeghzadeh |
Chemical & Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran

S. Ghazvini |
Chemical & Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran

S. Hejazi |
Chemical & Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran

S. Yaghmaei |
Chemical & Petroleum Engineering Department, Sharif University of Technology, Tehran, Iran

Z. Ghobadi Nejad |
Biochemical & Bioenvironmental Research Center, Sharif University of Technology, Azadi Avenue, Tehran, Iran

نشانی اینترنتی http://www.ije.ir/article_106022_684a8f217b2f636d5cbd3157c4ea4bcf.pdf
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