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Journal of Dentistry, Shiraz University of Medical Sciences، جلد ۲۴، شماره ۴، صفحات ۴۲۹-۴۳۷
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Molecular Identification of Candida albicans in Endodontic Retreatment Cases by SYBR Green I Real-time Polymerase Chain Reaction and its Association with Endodontic Symptoms |
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| چکیده انگلیسی مقاله |
Statement of the Problem: Recent microbiological studies have expressed ever-increasing concerns about Candida albicans as a causal factor in the failure of endodontic treatments. Real-time quantitative polymerase chain reaction (qPCR), including the SYBR Green I system, is a technique in which a fluorescent dye is incorporated into the double-stranded DNA that is produced during DNA polymerase activity.
Purpose: This study aimed to determine the relative prevalence of Candida albicans in root canals of retreatment cases and its association with endodontic symptoms.
Materials and Method: In the present cross-sectional/analytical study fifty subjects were selected. Clinical features and radiographic status of the teeth were also evaluated. After access cavity preparation, the retrieved material and dentinal chips removed from the root canal were transferred into 1.5-mL microtubes, followed by storage at -20ºC until used for DNA extraction. A DNeasy Tissue Kit was used to extract DNA using the DNeasy protocol for animal tissues. Master Plus SYBR Green I (Jena Bioscience, Germany) was used in a Rotor-gene Real-time PCR System for real-time PCR. The relationship between the presence of Candida albicans and the clinical and radiographic features were analyzed using McNemar’s test.
Results: There was a significant relationship between the radiographic findings in endodontically treated teeth and the presence of Candida albicans. However, there was no significant relationship between the presence of Candida albicans and any of the clinical symptoms.
Conclusion: In spite of the limitations of this study, we concluded that Candida albicans was associated with root canal infections in endodontic retreatment cases, but there was no relationship with the clinical symptoms. |
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| کلیدواژه‌های انگلیسی مقاله |
candida albicans,
Endodontics,
hsp60 protein,
Polymerase chain reaction |
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| نویسندگان مقاله |
Ahmad Nouroloyouni |
Dept. of Endodontics, Faculty of Dentistry, Ardabil University of Medical Sciences, Ardabil, Iran.
Negar Moghaddam |
Dept. of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences,Tabriz, Iran
Sarah Nouroloyouni |
Dept. of Pediatric Dentistry, Faculty of Dentistry, Ardabil University of Medical Sciences, Ardabil, Iran.
Amin Salem Milani |
Dept. of Endodontics, Dental and Periodontal Research Center, Dental Faculty, Golgasht Street, Tabriz University of Medical Sciences, Tabriz, Iran.
Hamidreza Yavary |
Dept. of Endodontics, Faculty of Dentistry, Tabriz University of Medical Sciences, Tabriz, Iran.
Alireza Majidi |
Dept. of Endodontics, Faculty of Dentistry, Qom University of Medical Sciences, Qom, Iran.
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| نشانی اینترنتی |
https://dentjods.sums.ac.ir/article_49202_5dfa042e1c010c6394a6b81252940f8f.pdf |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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