این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۵، شماره ۶، صفحات ۷۵۰-۷۵۸
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Comparison of culture and PCR-DGGE methods to evaluate the airways of cystic fibrosis patients and determination of their antibiotic resistance profile
چکیده انگلیسی مقاله Background and Objectives: Respiratory infections are the most serious condition in cystic fibrosis (CF) patients; therefore, a thorough comprehension of the diversity and dominant microbial species in CF airways has a crucial role in treatment. Our objective was to determine the antibiotic resistance profile of CF airways microbiota and compare culture methods and PCR-DGGE to evaluate bacterial diversity. Materials and Methods: Pharyngeal swabs from 121 CF patients were collected. The samples were then cultured, identified and antibiotic resistance testing was performed. Thirty samples were subjected to further molecular surveys. DNA contents of these samples were extracted and amplified using nested-PCR technique and their bacterial diversity was assessed by DGGE. The DGGE patterns were visualized and certain bands were excised and purified. Next, the DNA was amplified by another round of PCR and sent out for sequencing. Results: Staphylococcus aureus, Pseudomonas aeruginosa, and Klebsiella pneumoniae were the most prevalent species isolated using culture methods. S. aureus was the most common bacteria among 6 years and younger patients; while, P. aeruginosa had more prevalence among older ones. The PCR-DGGE results showed more diversity than culture methods, particularly in younger patients who exhibited more bacterial diversity than the older groups. Sequencing results unveiled the presence of certain bacterial species including Haemophilus parainfluenzae and Stenotrophomonas maltophilia which were completely missed in culture. Conclusion: Even though culture-dependent methods are cost-effective, PCR-DGGE appeared to be more efficient to determine bacterial diversity. PCR-DGGE detects less abundant species, though their viability could not be determined using this method.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Somayeh Moazami Goudarzi
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran; Cystic Fibrosis Research Center, Iran CF Foundation (ICFF), Tehran, Iran


| Yasamin Shahpouri Arani
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Ahya Abdi Ali
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Parisa Mohammadi
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Nassim Ghorbanmehr
Department of Biotechnology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Mohammadreza Modaresi
Cystic Fibrosis Research Center, Iran CF Foundation (ICFF), Tehran, Iran; Pediatric Pulmonary Disease and Sleep Medicine Research Center, Tehran University of Medical Sciences, Tehran, Iran


| Mahtab Ghorban Movahed
Department of Microbiology, Faculty of Biological Sciences, Alzahra University, Tehran, Iran


| Tooba Ghazanfari
Immunoregulation Research Center, Shahed University, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4155
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات