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Iranian Journal of Microbiology، جلد ۱۵، شماره ۵، صفحات ۶۶۵-۶۷۳
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عنوان انگلیسی Evaluation of miR-let-7f, miR-125a, and miR-125b expression levels in sputum and serum samples of Iranians and Afghans with pulmonary tuberculosis
چکیده انگلیسی مقاله Background and Objectives: The role of microRNAs (miRNAs) in tuberculosis infection is well established. As microRNAs are able to change expression profiles according to different conditions, they can be useful biomarkers. Iranians and Afghans with tuberculosis were studied for three immune-related miRNAs (miR-let-7f, miR-125a, and miR-125b). Materials and Methods: A total of 60 Iranian and Afghan patients with active pulmonary TB were enrolled in the Pulmonary Department of the Pasteur Institute of Iran. Serum and sputum samples were collected simultaneously from all participants. A Real-time PCR was conducted to detect differentially expressed miRNAs. Results: Iranian (P< 0.0001) and Afghan (P< 0.0001) serum samples and Afghan (P< 0.0001) sputum samples overexpressed miR-125a, whereas Iranian sputum samples showed downregulation (P=0.0039). In both Iranian (P< 0.0001; P=0.0007) and Afghan (P< 0.0001; P< 0.0001) serum and sputum samples, miR-125b was overexpressed. Furthermore, miR-let-7f downregulation was observed in serum and sputum samples (P< 0.0001), whereas Iranian sputum samples had no statistically significant differences (P=0.348). Conclusion: Overexpression of miR-125a and miR-125b has been detected in Iranian and Afghan samples. In both races, miR-let-7f downregulation has been confirmed. Identification of miRNA profiles under different conditions opens the door to evaluating potential new biomarkers for diagnosis, disease monitoring, and therapeutic markers in TB infection.
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نویسندگان مقاله | Ali Nour Neamatollahi
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran


| Samira Tarashi
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran


| Nayereh Ebrahimzadeh
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran


| Farzam Vaziri
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran


| Mohammad Ali Zaheri Birgani
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran


| Mohammadreza Aghasadeghi
Department of Hepatitis and AIDS, Pasteur Institute of Iran, Tehran, Iran; Viral Vaccine Research Center, Pasteur Institute of Iran, Tehran, Iran


| Abolfazl Fateh
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran


| Seyed Davar Siadat
Department of Mycobacteriology and Pulmonary Research, Pasteur Institute of Iran, Tehran, Iran; Microbiology Research Center (MRC), Pasteur Institute of Iran, Tehran, Iran


| Saeid Bouzari
Department of Molecular Biology, Pasteur Institute of Iran, Tehran, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/4301
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