این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Ecopersia، جلد ۱۱، شماره ۳، صفحات ۱۸۷-۱۹۵
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Influence of Enzyme Type and Hydrolysis Time on Antioxidant Activity of Hydrolyzed Protein from Longtail Tuna (Thunnus tonggol) Dark Muscle
چکیده انگلیسی مقاله Aim: In this study, the antioxidant properties of hydrolyzed protein from longtail tuna dark muscle with commercial enzymes (alcalase, alkaline protease, and evatase) were investigated. Materials & Methods: Protein hydrolysates from tuna dark muscle were prepared by different enzymes Degree of hydrolysis (DH) was performed by TCA technique. The five aliquots at 60, 180, 240, 300, and 360 min were gathered during hydrolysis. The antioxidant activity of aliquots was monitored by in vitro assays (DPPH inhibition ability and Ferric (Fe3+) reducing power). Findings: The antioxidant activities of protein hydrolysate from tuna dark muscle (TDM) increase with increasing time and DH. Alcalase hydrolyzed protein (AHP) generally showed higher antioxidative activity than evatase hydrolyzed protein (EHP) and alkaline protease hydrolyzed protein (APHP). Among the samples (concentration 3 mg.ml-1), AHP at 360 min significantly exhibited the highest ability to scavenge DPPH radical (72.6 %). Furthermore, AHP and APHP significantly showed a minimum IC50 value of 1.1 mg.ml-1 at 240 and 360 min hydrolysis. APHP significantly exhibited the highest ferric reducing power of 0.83 at 300 min and 0.76 at 240 min. AHP and APHP significantly showed the highest ferric reducing power of 0.74 at 360 min (p < 0.05). Conclusion: This study confirmed that protein hydrolysate from TDM could be a good source of antioxidant peptides. In addition, the antioxidant activity of hydrolyzed protein relay on protease type and hydrolysis condition.  
کلیدواژه‌های انگلیسی مقاله Antioxidant,enzyme,dark muscle,protein hydrolysate,Thunnus tonggol
نویسندگان مقاله | aliyeh daryanavard
Ph.D. student, marine biology department, faculty of natural resources and marine science, Tarbiat Modares University


| Saber Khodabandeh
associated professor, marine biology department, faculty of natural resources and marine science


| Reza Hassan Sajedi
Professor, Department of Biochemistry, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran


| Mehrdad Behmanesh
Professor, Department of Genetic, Faculty of Biological Sciences, Tarbiat Modares University, Tehran, Iran
نشانی اینترنتی http://ecopersia.modares.ac.ir/browse.php?a_code=A-10-45834-6&slc_lang=en&sid=24
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات