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Veterinary Research Forum، جلد ۷، شماره ۴، صفحات ۳۱۹-۳۲۴
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
Hydrogen sulfide upregulated mRNA expressions of sodium bicarbonate cotransporter1, trefoil factor1 and trefoil factor2 in gastric mucosa in rats |
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| چکیده انگلیسی مقاله |
Hydrogen sulfide (H2S) has been shown to protect the gastric mucosa through several protective mechanisms but till now its effect on mRNA expression of sodium bicarbonate cotransporter 1 (NBC1), trefoil factor1 (TFF1) and trefoil factor2 (TFF2) was not investigated. This study was aimed to evaluate the effect of H2S on mRNA expression of NBC1, TFF1 and TFF2 in rat gastric mucosa in response to gastric distention. Thirty two rats were randomly assigned into four equal groups. They were control (C), distention (D), propargylglycine (PAG)-, and NaHS-treated groups. To evaluate the effect of exogenous and endogenous H2S on gene expression of NBC1, TFF1 and TFF2, two groups of rats were received H2S donor, intra-peritoneal NaHS (80 µg Kg-1), and PAG (50 mg kg-1), accompanied to stimulate the gastric acid secretion, respectively. Under general anesthesia and laparotomy, a catheter was inserted into the stomach through duodenum for instillation of isotonic saline for gastric distention. Ninety min after beginning the experiment, animals were sacrificed and the gastric mucosa was collected to determine total acid content of gastric effluents and to quantify the mRNA expression of studied genes by quantitative real-time polymerase chain reaction (qRT-PCR). Results showed that A) gastric distention increased the level of mRNA expressions of NBC1, TFF1 and TFF2; B) these levels in NaHS-treated rats were significantly higher than those in Distention group; and C) PAG decreased the expression levels of NBC1 and TFF1. The Findings showed H2S upregulated gene expression of NBC1, TFF1 and TFF2 in gastric mucosa. |
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| نویسندگان مقاله |
پریسا چراغی |
department of molecular and cellular sciences, faculty of advanced sciences and technology, pharmaceutical sciences branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
سید علی مرد | seyyed ali
research center for infectious diseases of digestive system, physiology research center, department of physiology, school of medicine, ahvaz jundishapur university of medical sciences, ahvaz, iran
سازمان اصلی تایید شده: دانشگاه علوم پزشکی جندی شاپور اهواز (Ahvaz jundishapur university of medical sciences)
طاهره نقی |
department of molecular and cellular sciences, faculty of advanced sciences and technology, pharmaceutical sciences branch, islamic azad university, tehran, iran
سازمان اصلی تایید شده: دانشگاه آزاد اسلامی علوم و تحقیقات (Islamic azad university science and research branch)
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| نشانی اینترنتی |
http://vrf.iranjournals.ir/article_22158_5a547c3addfc657f9ca516ab2506d50b.pdf |
| فایل مقاله |
اشکال در دسترسی به فایل - ./files/site1/rds_journals/544/article-544-279629.pdf |
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| زبان مقاله منتشر شده |
en |
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