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Avicenna Journal of Medical Biotechnology، جلد ۱۵، شماره ۲، صفحات ۷۶-۸۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria |
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| چکیده انگلیسی مقاله |
Background: Stem cells are used to treat numerous diseases; however, their lifespan is rather short. Factors such as probiotics affect and improve various cell lineage efficacies. The aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis. Methods: The experiment was initiated by culturing Lactobacillus casei and Lactobacillus acidophilus probiotics as well as DPS-7 cells. Bacterial supernatants were separated and concentrated as the conditioned media. The DPS-7 cells were treated with various concentrations of the conditioned media. Furthermore, MTT assay and alkaline phosphatase activity were used. The mRNA expression of three genes (bFGF, EGF-β and BMP-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction. Results: The response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bFGF and BMP-2 gene expression. Increased expression was significant for BMP-2 and moderate for bFGF; however, it was non-significant for EGF-β. The use of the two probiotics was the most effective. Conclusion: In general, synergism of the combined probiotics preconditioning induces differentiation of DPS-7 cells into osteoblasts most effectively. |
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| کلیدواژه‌های انگلیسی مقاله |
Dental caries, Osteogenesis, Probiotics, Stem cells |
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| نویسندگان مقاله |
| Fatemeh Amini
School of Dentistry, Shahed University of Medical Sciences, Tehran, Iran
| Mohammad Bagher Rezvani
Department of Restorative Dentistry, School of Dentistry, Shahed University, Tehran, Iran
| Ronak Bakhtiari
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran
| Elham Tabatabaei Ghomsheh
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| نشانی اینترنتی |
https://www.ajmb.org/En/Article.aspx?id=60533 |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
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