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Avicenna Journal of Medical Biotechnology، جلد ۱۵، شماره ۲، صفحات ۷۶-۸۳
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عنوان انگلیسی Effects of Dental Pulp Stem Cell Preconditioning on Osteogenesis using Conditioned Media of Probiotics Bacteria
چکیده انگلیسی مقاله Background: Stem cells are used to treat numerous diseases; however, their lifespan is rather short. Factors such as probiotics affect and improve various cell lineage efficacies. The aim of this study was to investigate the effects of probiotics-conditioned media on dental pulp stem cell potentials in osteogenesis. Methods: The experiment was initiated by culturing Lactobacillus casei and Lactobacillus acidophilus probiotics as well as DPS-7 cells. Bacterial supernatants were separated and concentrated as the conditioned media. The DPS-7 cells were treated with various concentrations of the conditioned media. Furthermore, MTT assay and alkaline phosphatase activity were used. The mRNA expression of three genes (bFGF, EGF-β and BMP-2) involved in osteogenesis was analyzed using a real-time polymerase chain reaction. Results: The response of dental pulp stem cells to probiotics preconditioning promoted cell proliferation, increased alkaline phosphatase activity and upregulated bFGF and BMP-2 gene expression. Increased expression was significant for BMP-2 and moderate for bFGF; however, it was non-significant for EGF-β. The use of the two probiotics was the most effective. Conclusion: In general, synergism of the combined probiotics preconditioning induces differentiation of DPS-7 cells into osteoblasts most effectively.
کلیدواژه‌های انگلیسی مقاله Dental caries, Osteogenesis, Probiotics, Stem cells
نویسندگان مقاله | Fatemeh Amini
School of Dentistry, Shahed University of Medical Sciences, Tehran, Iran


| Mohammad Bagher Rezvani
Department of Restorative Dentistry, School of Dentistry, Shahed University, Tehran, Iran


| Ronak Bakhtiari
Department of Pathobiology, School of Public Health, Tehran University of Medical Sciences, Tehran, Iran


| Elham Tabatabaei Ghomsheh


نشانی اینترنتی https://www.ajmb.org/En/Article.aspx?id=60533
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