این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۴، شماره ۵، صفحات ۶۳۶-۶۴۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Genomic analysis of Fosfomycin resistance in multi-drug resistant uropathogens and comparison of in-vitro susceptibility methods uropathogens
چکیده انگلیسی مقاله Background and Objectives: Urinary tract infection is one of the most common bacterial infections causing high morbidity and mortality. The alarming rise of multidrug-resistant uropathogens worldwide forced the clinician to rethink the old drugs like Fosfomycin for its therapeutic management. Our objective was to compare agar dilution, disc diffusion and E-test method for antimicrobial susceptibility testing of Fosfomycin against different drug-resistant uropathogens. Materials and Methods: Consecutive 181 uropathogens were tested for Fosfomycin susceptibility using agar dilution, disc diffusion and E-test. Results were interpreted using Clinical and Laboratory Standards Institute (CLSI) and European Committee on Antimicrobial Susceptibility Testing (EUCAST) breakpoints. Whole genome sequencing analysis was done on the 4 XDR/PDR Fosfomycin resistant Klebsiella pneumoniae isolates. Results: Escherichia coli was found as the most common (62.4%) uropathogen followed by Klebsiella pneumoniae (21%). Considering agar dilution as the gold standard, 6.1% of isolates were resistant to Fosfomycin. Following CLSI breakpoints, the susceptibility of Escherichia coli, Klebsiella pneumoniae, other Enterobacterales and Pseudomonas aeruginosa were 92.9%, 92.1%, 100%, 100%; whereas using EUCAST breakpoints the susceptibility rates were 85.7%, 86.9%, 92.9%, and 100%, respectively. The essential agreement, categorical agreement, major error, and very major error for E-test/ disc diffusion for all the organisms were 91.2%/Not Applicable, 95%/93.9%, 1.8%/4.7%, 9.1%/9.1%, respectively. Whole-genome sequencing showed mutation UhpT gene as well as the presence of plasmid-mediated fosA5 or fosA6 genes conferring Fosfomycin resistance. Conclusion: This result supports very low resistance of Enterobacterales against Fosfomycin; hence should be considered a valuable option to treat multidrug-resistant uropathogens. Disc diffusion was observed to be a convenient method for Fosfomycin susceptibility testing compared to agar dilution.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Raunak Bir
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Sarita Mohapatra
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Arvind Kumar
Department of Medicine, All India Institute of Medical Sciences, New Delhi, India


| Nazneen Arif
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Sonu Tyagi
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Aswin AK
Department of Medicine, All India Institute of Medical Sciences, New Delhi, India


| Hitender Gautam
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Seema Sood
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Bimal Das
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India


| Arti Kapil
Department of Microbiology, All India Institute of Medical Sciences, New Delhi, India
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3500
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات