Avicenna Journal of Medical Biotechnology، جلد ۱۴، شماره ۴، صفحات ۲۹۴-۳۰۲
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عنوان انگلیسی Evaluation of PLGA-Encapsulated Recombinant GroEL of S. typhi immune Responses Against Enterohaemorrhagic and Enteropathogenic Escherichia coli
چکیده انگلیسی مقاله Background: Heat Shock Proteins (HSPs) elicit humoral and cellular immune responses. Due to their high sequence homology, they can be developed as a new immunogen for cross prophylactic and vaccination effects against infectious agents such as Enteropathogenic and Enterohemorrhagic Escherichia coli (EPEC and EHEC). This study aimed to evaluate the immunogenicity and cross-protective efficacy of rGroEL of Salmonella typhi (S. typhi) encapsulated in poly lactic-co-glycolic acid (PLGA) nanoparticles against EPEC and EHEC. Methods: Recombinant GroEL was expressed in Escherichia coli (E. coli) and purified using Ni-NTA affinity chromatography. The protein was encapsulated in PLGA by the double emulsion method, and the nanoparticles were characterized physicochemically. BALB/c mice were immunized, and the efficacy of the protein to elicit immune responses was assessed. Results: Over-expression in E. coli led to corresponding 64.5 kDa protein bands in Sodium Dodecyl Sulphate-Polyacrylamide Gel Electrophoresis (SDS-PAGE). Non-ag-gregated nanoparticles had a spherical shape with a mean diameter of 194.3±3 nm and encapsulation efficiency of 89.5±2.5%. Antibody isotyping revealed that GroEL immunization induced both IgG1 and IgG2a antibodies. Moreover, immunization of the mice with recombinant GroEL protein conferred 80 and 60% protection against lethal infections by EPEC and EHEC, respectively. Furthermore, organ burden studies revealed a significant reduction in infection in the immunized mice compared to the non-immunized ones. Passive immunization with anti-GroEL sera also protected 50% of the mice against the lethal doses of EHEC and EPEC strains. Conclusion: The findings indicated that immunization of the mice with recombinant GroEL of S. typhi elicited cross-protection against other bacterial infections. This represented the immense potential of GroEL to be developed as a single vaccine against multiple pathogens
کلیدواژه‌های انگلیسی مقاله Heat-shock proteins, Immunogenicity, Nanoparticles, Salmonella typhi, Vaccines
نویسندگان مقاله | Milad Parvane
Biology Research Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran


| Shahram Nazarian


| Emad Kordbacheh
Biology Research Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran


| Javad Fathi
Student Research Committee, School of Medicine, Shiraz University of Medical Sciences, Shiraz, Iran


| Mohamad Ebrahim Minae


| Mohammad Reza Ramezani
Biology Research Center, Faculty of Basic Sciences, Imam Hossein University, Tehran, Iran
نشانی اینترنتی https://www.ajmb.org/En/Article.aspx?id=60517
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