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Iranian Journal of Microbiology، جلد ۱۴، شماره ۳، صفحات ۳۹۵-۴۰۱

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عنوان انگلیسی Application of bacteriophage cocktails for reducing the bacterial load of nosocomial pathogens in hospital wastewater
چکیده انگلیسی مقاله Background and Objectives: In the third world and developing countries, hospital sewage is mixed with municipal wastewater. The treated effluent contains dangerous bacteria released into the environment and used in the irrigation of agricultural products, and eventually these bacteria may endanger the human health through foods. Antibiotic-resistant bacteria are mostly found in hospital wastewater. In water and wastewater treatment plants, large amounts of toxic and polluting substances are removed and destroyed, but this process does not eliminate bacteria. Materials and Methods: Wastewater samples from 22 hospitals in Iran were collected and in the meantime specific phages (against drug-resistant pathogenic bacteria) extracted using the bilayer agar technique. Phage amplification was performed by employing a fermenter after phage identification. Amplified phages were added to the primary sedimentation pond using New-Brunwick biofermenter BioFlo/Celligen®115 and the bacterial count was evaluated for the desired bacteria. Results: Our phage cocktail was able to reduce 99.8%, 99.4%, 99.5%, 99.8%, 99.7%, 99.8%, 99.6% and 99.9% of E. coli, E. faecium, E. faecalis, K. pneumoniae, A. baumannii, P. aeruginosa, S. maltophilia and S. aureus counts respectively. Conclusion: The application of phage cocktails can remarkably help improve personal hygiene, the environment, and the optimization of surface water.
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نویسندگان مقاله | Sara Sadeqi
Student Research Committee, Qazvin University of Medical Sciences, Qazvin, Iran


| Abdolrazagh Hashemi Shahraki
Division of Pulmonary, Miller School of Medicine, University of Miami, Miami, USA


| Farhad Nikkhahi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran


| Amir Javadi
Department of Community Medicine, Qazvin University of Medical Sciences, Qazvin, Iran


| Seyed Mohammad Amin Marashi
Medical Microbiology Research Center, Qazvin University of Medical Sciences, Qazvin, Iran



نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3247
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