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Research in Pharmaceutical Sciences، جلد ۱۷، شماره ۳، صفحات ۲۸۴-۲۹۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Design and production of a novel chimeric human growth hormone superagonist fused to human Fc domain |
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| چکیده انگلیسی مقاله |
Background and purpose: Growth hormone (GH) has been known as a crucial metabolic hormone expressed at the pituitary and the other number of cells and tissues and responsible for body growth. Because of the short half-life of GH, daily subcutaneous injections were shown to be more effective for GH therapy. This represents a burden for patients. So, there is a strong effort from the industry to create a long-acting form of GH and lots of technologies like GH fusion proteins are used to increase GH half-life. Experimental approach: In this study, the Fc domain of human IgG1 with serine-glycine linkers was attached to the C-terminal of a GH superagonist via molecular cloning. The presence of recombinant vector in E. coli host was confirmed by PCR. SDS-PAGE and western blot analysis showed the expression of recombinant proteins in the bacterial lysate. The binding ability to growth hormone receptors is determined by ELISA. Findings / Results: Our results showed that the novel SupGH-Fc has a good binding affinity to its receptor in ELISA in comparison to standard GH, although it has a big size. Conclusion and implications: Our data in this study clearly demonstrated the expression of the SupGH-Fc in a recombinant protein expression system. It is an introduction to the production of the new recombinant GH, which can bind to its receptor more effectively than commercial growth hormones and also might have a longer half-life. |
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| کلیدواژه‌های انگلیسی مقاله |
Fc fusion proteins,Growth hormone,Growth hormone superagonist,Long half-life. |
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| نویسندگان مقاله |
| Samira Mirbaha
2Department of Immunology, School of Medicine, Isfahan University of Medical Sciences, Isfahan, I.R. Iran.
| Marzieh Rezaei
1Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, I.R. Iran.
| Rahman Emamzadeh
1Department of Cell and Molecular Biology and Microbiology, Faculty of Biological Science and Technology, University of Isfahan, Isfahan, I.R. Iran.
| Sayyed Hamid Zarkesh Esfahani
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| نشانی اینترنتی |
http://rps.mui.ac.ir/index.php/jrps/article/view/2126 |
| فایل مقاله |
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| کد مقاله (doi) |
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| زبان مقاله منتشر شده |
en |
| موضوعات مقاله منتشر شده |
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| نوع مقاله منتشر شده |
Original Article |
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