Avicenna Journal of Medical Biotechnology، جلد ۱۴، شماره ۲، صفحات ۱۳۲-۱۳۶
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عنوان انگلیسی The Inhibitory Effect of Thioridazine on adeB Efflux Pump Gene Expression in Multidrug-Resistant Acinetobacter baumannii Isolates Using Real Time PCR
چکیده انگلیسی مقاله Background: The purpose of the present study was to investigate the antimicrobial effects of berberine and thioridazine, as well as their effect on the gene expression of the AdeABC efflux pump system in Multidrug-Resistant (MDR) Acinetobacter baumannii (A. baumannii) isolates. Methods: This study was carried out in five MDR clinical isolates of A. baumannii and a sample of standard strain (A. baumannii PTCC1797). The effect of Minimum Inhibitory Concentration (MIC) and the Minimum Bactericidal Concentration (MBC) of berberine, thioridazine, and ciprofloxacin alone and their combination on A. baumannii was evaluated by broth microdilution method. Also, their effect on the expression of adeB efflux pump gene was evaluated using real time PCR method.  Results: The MIC of thioridazine, berberine, ciprofloxacin+thioridazine, ciprofloxacin+berberine, thioridazine+berberine, and ciprofloxacin+thioridazine+berberine on MDR A. baumannii isolates was 64, 256, 128, 256, 128, and 128 μg/ml, respectively. The results showed that treatment of strains with thioridazine alone and in combination with berberine and ciprofloxacin significantly (p< 0.05) decreased the expression of adeB efflux pump gene. Conclusion: Due to the inhibitory effects of thioridazine on bacterial isolates and adeB efflux pump gene, this compound can be used as a potential antimicrobial agent against MDR A. baumannii.
کلیدواژه‌های انگلیسی مقاله Acinetobacter baumannii, Ciprofloxacin, Gene expression, Real-Time Polymerase Chain Reaction, Thioridazine
نویسندگان مقاله | Fereshteh Ahmadi
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran


| Bahman Khalvati
Medicinal Plants Research Center, Yasuj University of Medical Sciences, Yasuj, Iran


| Saba Eslami
Shiraz University of Medical Sciences, Shiraz, Iran


| Mehdi Mirzaii
Faculty of Medicine, Shahroud University of Medical Sciences, Shahroud, Iran


| Narges Roustaei
Department of Epidemiology and Biostatistics, School of Health and Nutrition Sciences, Social Determinants of Health Research Center, Yasuj University of Medical Sciences, Yasuj, Iran


| Farzad Mazloomirad
Student Research Committee, Yasuj University of Medical Sciences, Yasuj, Iran


| Seyed Sajjad Khoramrooz
Department of Microbiology, School of Medicine, Yasuj University of Medical Sciences, Yasuj, Iran
نشانی اینترنتی https://www.ajmb.org/En/Article.aspx?id=60495
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