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Avicenna Journal of Medical Biotechnology، جلد ۱۳، شماره ۱، صفحات ۴۲-۴۶
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
β-sitosterol Mediated Silver Nanoparticles Induce Cytotoxicity in Human Colon Cancer HT-29 Cells |
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| چکیده انگلیسی مقاله |
Background: Silver nanoparticles (AgNP) are commonly used metallic nanoparticles in health care systems. Colon cancer incidence is increasing worldwide. In this study, AgNP was synthesized using β-sitosterol and its cytotoxic potential was evaluated in human colon cancer (HT-29) cells. Methods: Characterization of AgNP was analyzed by TEM and spectrophotometry analysis. HT-29 cells were treated with different concentrations (2, 4, 6, 8 and 10 ng/ml) of AgNPs and cytotoxicity was evaluated by MTT assay. The apoptosis was analyzed by the flow cytometry. The expression of p53 protein was analyzed by western blotting. Results: β-sitosterol mediated AgNP are spherical in shape and induced concentration-dependent cytotoxicity in HT-29 cells. AgNP caused apoptosis related morphological changes as evidenced by annexin positive staining. AgNP treatments also induced the p53 expression in HT-29 cells. Conclusion: Our present result suggests that β-sitosterol mediated AgNP induce apoptosis in colon cancer cells and this finding may pave the way for further experimental analysis in vivo. |
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| کلیدواژه‌های انگلیسی مقاله |
Apoptosis, Colon neoplasms, HT29 cells, Silver nanoparticles, β-sitosterol |
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| نویسندگان مقاله |
| Palaniappan Chithambara Shathviha
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai, Tamil Nadu, India
| Devaraj Ezhilarasan
| Shanmugam Rajeshkumar
Department of Pharmacology, Saveetha Dental College, Saveetha Institute of Medical and Technical Sciences (SIMATS), Chennai,, Tamil Nadu, India
| Jayaraman Selvaraj
Department of Biochemistry, Saveetha Dental College (SDC), Saveetha Institute of Medical and Technical Sciences, Chennai, Tamil Nadu, India
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| نشانی اینترنتی |
https://www.ajmb.org/En/Article.aspx?id=40446 |
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| زبان مقاله منتشر شده |
en |
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