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Avicenna Journal of Medical Biotechnology، جلد ۱۳، شماره ۲، صفحات ۹۸-۱۰۳
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| عنوان انگلیسی |
The Effect of Silver Nanoparticles on Pyocyanin Production of Pseudomonas aeruginosa Isolated From Clinical Specimens |
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| چکیده انگلیسی مقاله |
Background: Pseudomonas aeruginosa (P. aeruginosa) is an opportunistic pathogen causing a wide range of human infections. The organism is resistant to a wide range of antibiotics. The purpose of this study was to investigate the effect of AgNPs on pyocyanin pigment production of P. aeruginosa bacteria isolated from clinical specimens. Methods: In this study, 15 clinical isolates of P. aeruginosa were collected from different specimens of hospitalized patients. P. aeruginosa was detected by biochemical and molecular (detection of pbo1 gene by colony PCR method) methods and the MIC and MBC of AgNPs were determined by agar dilution method. Inhibition of P. aeruginosa pyocyanin production at AgNPs concentrations of 0, 0.3, 0.5, 1 and 1.5 mg/ml of was studied with OD of 520 nm. Results: The mean MIC and MBC of AgNPs were 1.229 and 1.687 mg/ml, respectively. Pyocyanin production was investigated for all isolates at different concentrations of nanoparticles, and their comparison showed that with increasing nanoparticle concentration, pyocyanin production significantly decreased (p< 0.05). Conclusion: According to the results of this study, AgNPs had an inhibitory effect on P. aeruginosa and its pigment production and with increasing nanoparticles concentration, pigment production decreased; therefore, it seems that the nanoparticles can be used to treat and prevent diseases caused by P. aeruginosa. |
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| کلیدواژه‌های انگلیسی مقاله |
Nanoparticles, Polymerase chain reaction , Pseudomonas aeruginosa, Pyocyanin |
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| نویسندگان مقاله |
| Mahboobeh Najafi
Department of Biology, Faculty of Science, Damghan Branch, Islamic Azad University, Damghan, Iran
| Mahboobeh Nakhaei Moghaddam
Department of Biology, Faculty of Science, Mashhad Branch, Islamic Azad University, Mashhad, Iran
| Ehsan Yousefi
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| نشانی اینترنتی |
https://www.ajmb.org/En/Article.aspx?id=40457 |
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| زبان مقاله منتشر شده |
en |
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