Avicenna Journal of Medical Biotechnology، جلد ۱۴، شماره ۱، صفحات ۳۷-۴۵
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عنوان انگلیسی Monoclonal Antibody Against Sortilin Induces Apoptosis in Human Breast Cancer Cells
چکیده انگلیسی مقاله Background: Sortilin has an important role in various malignances and can be used as a promising target to eradicate cancer cells. Methods: In this study, the expression of sortilin in 4T1 and MDA-MB231 cell lines was evaluated by flow cytometry and immunocytochemistry. Apoptosis assay was also applied to evaluate apoptosis induction in 4T1 and MDA-MB231 cell lines. Results: Based on cell surface flow cytometry results, anti-sortilin (2D8-E3) mAb could recognize sortilin molecules in 79.2% and 90.3% of 4T1 and MDA-MB231 cell-lines, respectively. The immunocytochemistry staining results confirmed sortilin surface expression. Apoptosis assay indicated that anti-sortilin mAb could induce apoptosis in 4T1 and MDA-MB231 cell lines. Conclusion: Our study revealed the important role of surface sortilin in breast carcinoma cell survival and its possible application as a therapeutic agent in cancer targeted therapies.
کلیدواژه‌های انگلیسی مقاله Breast cancer, Flow cytometry, Monoclonal antibody, Sortilin
نویسندگان مقاله | Miganoosh Simonian
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Mozhan Haji Ghaffari
Department of Medical Biotechnology, School of Advanced Technologies in Medicine, Tehran University of Medical Sciences, Tehran, Iran


| Ali Salimi
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Ebrahim Mirzadegan
Nanobiotechnology Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Niloufar Sadeghi
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Nasim Ebrahimnezhad
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Ghazaleh Fazli
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Ramina Fatemi
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Ali Ahmad Bayat
Monoclonal Antibody Research Center, Avicenna Research Institute, ACECR, Tehran, Iran


| Saeideh Milani
Blood Transfusion Research Center, High Institute for Research and Education in Transfusion Medicine, Tehran, Iran


| Babak Negahdari


| Hodjattallah Rabbani


نشانی اینترنتی https://www.ajmb.org/En/Article.aspx?id=40492
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