Journal of Reproduction and Infertility، جلد ۲۱، شماره ۴، صفحات ۲۵۹-۲۶۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی The Effect of Preincubation Time and Myo-inositol Supplementation on the Quality of Mouse MII Oocytes
چکیده انگلیسی مقاله Background: It is demonstrated that optimal preincubation time improves oocyte quality, fertilization potential and developmental rate. This study aimed to evaluate the effect of preincubation time in the simple and myo-inositol supplemented medium on the oocyte quality regarding oxidative stress and mitochondrial alteration. Methods: Cumulus oocyte complexes (COCs) retrieved from superovulated NMRI mice were divided in groups of 0, 4 and 8 hr preincubation time in the simple and 20 mmol/L myo-inositol supplemented media. Intracellular reactive oxygen species (H2O2), glutathione (GSH), mitochondrial membrane potential (MMP), ATP content, and mitochondrial amount were measured and analyzed in experimental groups. One-way ANOVA and Kruskal-Wallis were respectively used for parametric and nonparametric variables. Statistical significance was defined as p< 0.05. Results: In comparison to control group, variables including ROS, GSH, mitochondrial amount, fertilization and developmental rates were significantly changed after 4 hr of preincubation in the simple medium, while MMP decreased following 8 hr of preincubation in the simple medium (p˂0.001). Preincubation of oocytes up to 8 hr in the simple medium could not decrease ATP content. For both 4 and 8 hr preincubation times, myo-inositole could decrease H2O2 and increase GSH and MMP levels and consequently could improve fertilization rate compared to oocytes preincubated in the simple culture. Conclusion: It seems that 4 hr or more preincubation time can decrease the oocyte quality and lead to reduced oocyte fertilization and developmental potential. Howevere, myo-inositol may prevent oocyte quality reduction and improve fertilization potential in comparision to the equivalent simple groups.
کلیدواژه‌های انگلیسی مقاله Developmental rate, Fertilization potential, Mitochondrial alteration, Myo-inositol supplement, Oocyte preincubation time, Oocyte quality, Oxidative stress
نویسندگان مقاله | Fatemeh Mohammadi
Anatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran


مهناز اشرفي | Mahnaz Ashrafi
Cellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran


| Zahra Zandieh
Cellular and Molecular Research Center, Iran University of Medical Sciences (IUMS), Tehran, Iran


| Mohammad Najafi
Biochemistry Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran


| Behrooz Niknafs
Anatomy Department, School of Medicine, Tabriz University of Medical Sciences, Tabriz, Iran


| Fatemeh Sadat Amjadi
Shahid Akbar Abadi Clinical Research Development Unit (ShACRDU), Iran University of Medical Sciences (IUMS), Tehran, Iran


| Maryam Haghighi
Anatomy Department, School of Medicine, Iran University of Medical Sciences (IUMS), Tehran, Iran
نشانی اینترنتی https://www.jri.ir/article/120086
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده en
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات