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Journal of Reproduction and Infertility، جلد ۲۲، شماره ۲، صفحات ۸۵-۹۲
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| عنوان فارسی |
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| چکیده فارسی مقاله |
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| کلیدواژه‌های فارسی مقاله |
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| عنوان انگلیسی |
Characterization of DDX4 Gene Expression in Human Cases with Non-Obstructive Azoospermia and in Sterile and Fertile Mice |
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| چکیده انگلیسی مقاله |
Background: In mammals, spermatogenesis is the main process for male fertility that is initiated by spermatogonial stem cells (SSCs) proliferation. SSCs are unipotent progenitor cells accountable for transferring the genetic information to the following generation by differentiating to haploid cells during spermato-and spermiogenesis. DEAD-box helicase 4 (DDX4) is a specific germ cell marker and its expression pattern is localized to, spermatocytes, and spermatids. The expression in the SSCs on the basement membrane of the seminiferous tubules is low. Methods: Immunohistochemistry (IHC) and Fluidigm reverse transcriptase-polymerase chain reaction (RT-PCR) were used to analyze the expression of DDX4 in testis tissue of fertile and sterile mice and human cases with non-obstructive azoospermia. Results: Our immunohistochemical findings of fertile and busulfan-treated mice showed expression of DDX4 in the basal and luminal compartment of seminiferous tubules of fertile mice whereas no expression was detected in busulfan-treated mice. The immunohistochemical analysis of two human cases with different levels of non-obstructive azoospermia revealed more luminal DDX4 positive cells. Conclusion: Our findings indicate that DDX4 might be a valuable germ cell marker for analyzing the pathology of germ cell tumors and infertility as global urological problems. |
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| کلیدواژه‌های انگلیسی مقاله |
DDX4 protein, Seminiferous tubules, Spermatogonial stem cell, Testicles |
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| نویسندگان مقاله |
| Hossein Azizi
Department of Nanobiotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
| Amirreza NiaziTabar
Department of Nanobiotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
| Atiyeh Mohammadi
Department of Nanobiotechnology, Faculty of Biotechnology, Amol University of Special Modern Technologies, Amol, Iran
| Thomas Skutella
Institute for Anatomy and Cell Biology, Medical Faculty, University of Heidelberg, Heidelberg, Germany
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| نشانی اینترنتی |
https://www.jri.ir/article/120102 |
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| زبان مقاله منتشر شده |
en |
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