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پژوهش‌های آسیب‌ شناسی زیستی، جلد ۲۴، شماره ۱، صفحات ۴۵-۵۴
عنوان فارسی پروتوکل ساخت ویروس سرخک از توالی آنتی ژنومیک نوترکیب و غیر نو ترکیب
چکیده فارسی مقاله   Measles virus, negative-strand RNA viruses, has been known as an ideal candidate in oncolytic virotherapy.  Recombinant measles virus can encode genes of interests for reaching several aims. Replication efficiency of oncolytic virus in tumoral cells is a key parameter in efficient tumor eradication. Products of P gene (P/V/C) support measle virus to circumvent IFN 1 as the main response of innate immune system against viruses. But vaccine strains used in oncolytic therapy studies comprise several mutations in their P gene sequences. These mutations affect replication efficacy which cause attenuation of measles strains applicable in vaccination. So, arming vaccine strains with the wild type P gene is helpful to reach high virus titer. Here at this study, we have expanded a protocol with details for engineering and efficient recovery of measles virus for different aims.   
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Protocol for Recovery of Measles Virus from Intact/Recombinant Antigenomic DNA
چکیده انگلیسی مقاله Measles virus, negative-strand RNA viruses, has been known as an ideal candidate in oncolytic virotherapy.  Recombinant measles virus can encode genes of interests for reaching several aims. Replication efficiency of oncolytic virus in tumoral cells is a key parameter in efficient tumor eradication. Products of P gene (P/V/C) support measle virus to circumvent IFN 1 as the main response of innate immune system against viruses. But vaccine strains used in oncolytic therapy studies comprise several mutations in their P gene sequences. These mutations affect replication efficacy which cause attenuation of measles strains applicable in vaccination. So, arming vaccine strains with the wild type P gene is helpful to reach high virus titer. Here at this study, we have expanded a protocol with details for engineering and efficient recovery of measles virus for different aims.      
کلیدواژه‌های انگلیسی مقاله Key Words, Measles Virus,Reverse Genetics,Viral Rescue,Oncolytic Virotherapy
نویسندگان مقاله | roghayeh alizadeh
Baghyatollah Medical Sciences University


| Kayhan Azadmanesh
Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran


| Arash Arashkia
Department of Molecular Virology, Pasteur Institute of Iran, Tehran, Iran


| Mostafa Ghanei
Baghyatollah Medical Sciences University,Chemical Injuries Research Center
نشانی اینترنتی http://mjms.modares.ac.ir/browse.php?a_code=A-10-63339-2&slc_lang=en&sid=30
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