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عنوان انگلیسی Evaluation of ku gene as a differential biomarker for rapid molecular detection of Mycobacterium tuberculosis complex using a real-time PCR assay
چکیده انگلیسی مقاله Purpose: currently TB diagnosis is limited by some major limitations in low-income and less experienced hospitals. Recently, it has been proposed that the ku gene of mycobacterial strains has the potential to be a highly specific and sensitive candidate biomarker for molecular detection of Mycobacterium tuberculosis (Mtb). This study was aimed to evaluate the specificity and sensitivity of a real-time PCR assay for detection of ku gene in Mtb complex to determine its applicability for Mtb identification. Materials and methods: The identification of Mtb was confirmed using GeneXpert assay. Specific primers for ku gene were designed and the cycle threshold (Ct) value from the real-time PCR was used as a proxy measure of the cut-off point. Receiver operating characteristic (ROC) curve analysis was conducted to determine the diagnostic performance of ku gene in detecting Mtb directly from clinical specimens. Results: ku amplification was interpreted as positive and negative based on Ct values, in which a value < 38 was considered positive and a value >40 was considered negative. Our findings revealed that the ku gene was found to be distributed in all Mtb-positive samples. Of note, none of the Mtb-negative exhibited a specific signal in a maximum of 40 cycles. Conclusions: The ku gene amplification using real-time PCR indicated high sensitivity and specificity for the detection of Mtb complex in sputum samples.
کلیدواژه‌های انگلیسی مقاله Mycobacterium tuberculosis,ku gene,biomarker,nucleic acid amplification tests (NAATs),GeneXpert MTB/RIF
نویسندگان مقاله | Tooba Radaei
Ph.D. student in Medical Bacteriology, Department of Bacteriology, Faculty of Medical Sciences, Tarbiat Modares University, Tehran, Iran


| Bita Bakhshi
Professor in Medical BacteriologyFaculty of Medical SciencesTarbiat Modares UniversityTehran, Iran


| Maryam Nikkhah
Department of Nanobiotechnology, Faculty of Biological Sciences, Tarbiat Modares University Tehran, Iran


| Gholamreza Hamzehloo
Tehran Regional Reference Laboratory for Tuberculosis, Tehran, Iran
نشانی اینترنتی http://mjms.modares.ac.ir/browse.php?a_code=A-10-67091-1&slc_lang=en&sid=30
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