این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Basic and Clinical Cancer Research، جلد ۱۲، شماره ۲، صفحات ۵۶-۶۹
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Investigation of MRP1 and ABCG2 Gene Expression in Chronic Myeloid Leukemia (CML) Patients
چکیده انگلیسی مقاله Background: This study evaluated and compared the quantitative expression of Multidrug Resistance-associated Protein 1 (MRP1) and ATP Binding Cassette subfamily G member 2 ( ABCG2), two Multidrug Resistance (MDR) related genes, in 30 CML patients and 27 normal subjects. Methods: Total RNA was extracted from peripheral blood Mononuclear cells (MNCs) using the Trizol method. Then cDNAs were synthesized. Gene expression was quantified with Real-Time PCR System. Relative expression of target genes was calculated using the 2-ΔΔCt method. Results: High expression of MRP1 and ABCG2 mRNAs were detected in the patient's group. Intra-group comparisons also revealed increased expression of ABCG2 in Accelerated Phase (AP)-Blastic Crisis (BC) patients compared to Chronic Phase (CP) patients. Whereas, increased expression of MRP1 in AP-BC patients was not statistically significant. Conclusion: Considering the wide spectrum of (ATP Binding Cassette) ABC transporter superfamily substrates, they can play an important role in cell fate determination. High expression of MRP1 and ABCG2 genes can result in the efflux of therapeutic agents, and subsequent reduction in their intracellular concentration. This mechanism finally protects the cells from the therapeutic effects of medications. On the other hand, these transporters are able to export growth factors out of the cell. Such exported molecules may have a growth-inducing effect on adjacent cells. These are the possible mechanisms for the participation of MRP1 and ABCG2 genes in conferring drug resistance to CML cells.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Saeed Solali
Hematology & Oncology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AND Molecular Medicine Research Center, Tabriz University of Medical Sciences, Tabriz, Iran.


| Masoumeh Fardi
Student Research Committee, Tabriz University of Medical Sciences, Tabriz, Iran. AND Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AND Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran


| Shohreh Almasi
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AND Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran.


| Mohammad Reza Aliparasti
Immunology Research Center, Tabriz University of Medical Sciences, Tabriz, Iran. AND Department of Immunology, Tabriz University of Medical Sciences, Tabriz, Iran.
نشانی اینترنتی https://bccr.tums.ac.ir/index.php/bccrj/article/view/358
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات