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Journal of Kerman University of Medical Sciences، جلد ۲۷، شماره ۴، صفحات ۳۱۸-۳۲۸
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عنوان انگلیسی A Novel in vitro Co-culture Systems on Differentiation of Embryonic Stem Cells into Oocyte-like Cells in an in vivo Manner
چکیده انگلیسی مقاله Background:Differentiation of Embryonic Stem Cells into Oocyte-like cells in vitro is challenging. Successful derivation of oocyte from stem cells can provide an alternative source for curing ovogenesis problems. The current study aims to demonstrate a new protocol with two different types of media for differentiating embryonic stem cells (ESCs) into oocyte-like cells (OLCs). Methods: After culturing mouse ESCs, embryoid bodies (EBs) were generated from ESCs by hanging drop (HD) method. To final differentiation of oocyte-like cells (OLCs), the EBs were cultured in two different types of media for 12 days (first 7 days EBs were cultured in in vitro maturation diluted in Granulose Cell- Conditioned Medium and Follicular Fluid [1:1:1] followed by 5 days of culture in in vitro maturation diluted in uterine condition medium [1:1] ). Results:According tothe MTT test, the viability rate increased in the experimental group compared to the control EBs cultured alone. Expression of Oct4, as a pluripotency marker, decreased during the differentiation process of EBs in the experimental group. Co-culturing of EBs with our mentioned protocol increased germ cell markers (Stella and Mvh) and increased Oocyte-specific markers (ZP1, Figα and GDF9). Conclusion:Our study introduces a promising in vitro protocol for achieving successful oogenesis through creating interactions of EBs with granulosa cells and uterine condition medium.
کلیدواژه‌های انگلیسی مقاله Embryonic Stem Cells, Conditioned Medium, Embryoid Bodies, in vitro maturation
نویسندگان مقاله Ali Delbari |
Assistant Professor, Cellular and Molecular Research Center & Department of Anatomical Sciences, School of Medicine, Sabzevar University of Medical Sciences, Sabzevar, Iran

Maryam Nazm Bojnordi |
Assistant Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Sina Mojaverrostami |
Department of Anatomy, School of Medicine, Tehran University of Medical Sciences, Tehran, Iran

Hatef Ghasemi Hamidabadi |
Associate Professor, Immunogenetic Research Center & Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

Zahra Bagheri‑Hosseinabadi |
Assistant Professor, Department of Clinical Biochemistry, School of Medicine, Rafsanjan University of Medical Sciences, Rafsanjan, Iran 6- Associate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University

Nourollah Rezaei |
Associate Professor, Immunogenetic Research Center& Department of Anatomy and Cell Biology, Faculty of Medicine, Mazandaran University of Medical Sciences, Sari, Iran

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_91018_f44b9d540fea885c20ab85d716ee7f6c.pdf
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