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Journal of Kerman University of Medical Sciences، جلد ۲۷، شماره ۵، صفحات ۴۱۴-۴۲۵
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عنوان انگلیسی Evaluation of the Cytotoxicity, Antibacterial, Antioxidant, and Anti-inflammatory Effects of Different Extracts of Punica granatum var. pleniflora
چکیده انگلیسی مقاله Background:Punica granatum var. pleniflora (PGP) has been used for thousands of years as an effective agent to treat various types of diseases. However, there are a few new evidences addressing its therapeutic effects and related mechanisms. Therefore, the aim of this study was to investigate the cytotoxic, antioxidant, antibacterial, and anti-inflammatory effects of ethanolic (ET), dichloromethane (DM), and ethyl acetate (EA) extracts of PGP. Methods: ET, DM, and EA extracts of PGP were first prepared using maceration method. Total phenolic content (TPC) of PGP was then assessed by the Folin-Ciocalteu assay, and its antioxidant capacity was determined by DPPH and FRAP methods. Furthermore, in-vitro antibacterial activity of the PGP extracts was performed. The effect of PGP on the viability of J774A.1, HUVECs, HT29, and MCF-7 cell lines was evaluated by the MTT assay. The anti-inflammatory effect of PGP was assessed in the lipopolysaccharide (LPS)-stimulated J774A.1 cell line using qRT-PCR method. Results: EA extract contained the highest phenolic content (383.3 ± 9.1 mg gallic acid/g extract) and showed the highest antioxidant activity (IC50 = 36.5 ± 2.3 µg/mL). PGP at concentration of 15 µg/mL significantly decreased the expression of COX-2 (ET) and iNOS (ET and EA) in J774A.1 cell. Also, EA showed the highest antibacterial activity. Furthermore, the PGP extracts decreased the viability of all tested cell lines in a concentration-dependent manner. As indicated by IC50, EA demonstrated the lowest IC50 for all tested cell lines. Conclusion: According to the results, antioxidant, anti-inflammatory, antibacterial, and cytotoxic effects of PGP might be driven by its phenolic compounds highly presented in the EA extract.
کلیدواژه‌های انگلیسی مقاله Punica granatum var. pleniflora Anti, inflammation Antioxidant Antibacterial Cytotoxicity Murine macrophage J774A.1
نویسندگان مقاله Mehrnaz Mehrabani |
Assistant Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

Mahboobeh Raeiszadeh |
Assistant Professor, Department of Traditional Pharmacy, Faculty of Traditional Medicine, Kerman University of Medical sciences, Kerman, Iran

Hamid Najafipour |
Professor, Physiology Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

Mojde Esmaeli Tarzi |
Cardiovascular Research Center, Institute of Basic and Clinical Physiology Sciences, Kerman University of Medical Sciences, Kerman, Iran

Arian Amirkhosravi |
Ph.D. Student, Pharmaceutical Sciences and Cosmetic Products Research Center, Kerman University of Medical Sciences, Kerman, Iran

Alireza Poustforoosh |
Ph.D. Student, Chemical Engineering Department, Faculty of Engineering, Shahid Bahonar University of Kerman, Kerman, Iran

Mohammad Ali Mohammadi |
Ph.D. Student, Research Center for Hydatid Disease in Iran, School of Medicine, Kerman University of Medical Sciences, Kerman, Iran

Sara Naghdi |
Student Research Committee, Kerman University of Medical Sciences, Kerman, Iran

Mitra Mehrabani |
Professor, Herbal and Traditional Medicines Research Center, Kerman University of Medical Sciences, Kerman, Iran

نشانی اینترنتی http://jkmu.kmu.ac.ir/article_91474_96cdafb062c30f332bc1b2f421cc4312.pdf
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