این سایت در حال حاضر پشتیبانی نمی شود و امکان دارد داده های نشریات بروز نباشند
Iranian Journal of Microbiology، جلد ۱۲، شماره ۴، صفحات ۲۹۶-۳۰۴
عنوان فارسی
چکیده فارسی مقاله
کلیدواژه‌های فارسی مقاله
عنوان انگلیسی Clinical and microbial characterization of toxigenic Clostridium difficile isolated from antibiotic associated diarrhea in Egypt
چکیده انگلیسی مقاله Background and Objectives: Clostridium difficile infection (CDI) has become a significant healthcare-associated infection throughout the world and is particularly important in developing countries. This study aimed to investigate clinical characterization and risk factors related to toxigenic C. difficile infection in adult and pediatric patients, antimicrobial susceptibility pattern. Also, to evaluate different diagnostic methods for rapid detection of C. difficile associated diarrhea (CDAD) in Egypt. Materials and Methods: Stool samples were collected from 95 pediatric patients and 37 adult patients suffering from antibiotic associated diarrhea and were subjected to direct toxin immunoassay and culture on cycloserine/cefoxitin/fructose agar. The presence of tcdA and tcdB genes was tested by PCR. Results: Toxigenic C. difficile was isolated from pediatric and adult patients at a rate of 17.89% (17/95) and 27% (10/37) respectively. The sensitivity and specificity of direct PCR from stool are (100%, 100% and 82.4%, 100%) in adult and pediatric samples respectively. The susceptibility of C. difficile to vancomycin and metronidazole were found to be 66.7% and 48.2% respectively. Conclusion: Diabetes mellitus, prior antibiotic treatment, hematological malignancy on chemotherapy, malnutrition, neutropenia and Ryle feeding are risk factors for development of CDAD. Tight restriction of unnecessary antibiotic uses is necessary in our locality. Direct detection of toxin genes in stool by PCR is sensitive and specific method for early detection of C. difficile.
کلیدواژه‌های انگلیسی مقاله
نویسندگان مقاله | Sherein Elgendy
Department of Medical Microbiology and Immunology, School of Medicine, Assiut University, Assiut, Egypt


| Sherine Aly
Department of Medical Microbiology and Immunology, School of Medicine, Assiut University, Assiut, Egypt


| Rawhia Fathy
Department of Medical Microbiology and Immunology, School of Medicine, Assiut University, Assiut, Egypt


| Enas Deaf
Department of Medical Microbiology and Immunology, School of Medicine, Assiut University, Assiut, Egypt


| Naglaa Abu Faddan
Department of Pediatric Medicine, Assiut University Hospitals, Assiut University, Assiut, Egypt


| Muhamad Abdel Hameed
Department of Internal Medicine, Hematology Unit, Assiut University Hospitals, Assiut University, Assiut, Egypt
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/2619
فایل مقاله فایلی برای مقاله ذخیره نشده است
کد مقاله (doi)
زبان مقاله منتشر شده
موضوعات مقاله منتشر شده
نوع مقاله منتشر شده
برگشت به: صفحه اول پایگاه   |   نسخه مرتبط   |   نشریه مرتبط   |   فهرست نشریات