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Iranian Journal of Microbiology، جلد ۱۳، شماره ۵، صفحات ۶۶۴-۶۷۰
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عنوان انگلیسی Cloning of Bacillus subtilis phytase gene construct in Escherichia coli
چکیده انگلیسی مقاله Background and Objectives: Phytase has a hydrolysis function of phytic acid, which yields inorganic phosphate. Bacillus species can produce thermostable alkaline phytase. The aim of this study was to isolate and clone a Phytase gene (Phy) from Bacillus subtilis in Escherichia coli. Materials and Methods: In this study, the extracellular PhyC gene was isolated from Bacillus subtilis Phytase C. After purification of the bands, DNA fragment of Phy gene was cloned by T/A cloning technique, and the clone was transformed into Escherichia coli. Afterward, the pGEM-Phy was transferred into E. coli Top-10 strain and the recombinants were plated on LB agar containing 100 µg/ml ampicillin. The colonization of 1171 bp of gene Phytase C was confirmed by PCR. The presence of gene-targeting in vector was confirmed with enzymatic digestion by XhoI and XbaI restriction enzymes. Results: The Phytase gene was successfully cloned in E. coli. The result of cloning of 1171 bp Phytase gene was confirmed by PCR assay. Conclusion: Our impression of this article is that several methods, such as using along with microbial, plant phytase reproduction, or low-phytic acid corn may be the better way from a single phytase.
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نویسندگان مقاله | Mahdiyar Iravani Saadi
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Abbas Doosti
Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran


| Heeva Jalali
Department of Animal Science, Faculty of Agriculture, University of Kurdistan, Sanandaj, Iran


| Ehsan Nabi Abdolyousefi
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran


| Mansooreh Hooshiyar
Department of Microbiology and Microbial Biotechnology, Faculty of Life Sciences and Biotechnology, Shahid Beheshti University, Tehran, Iran


| Reza Tabrizi
Non-Communicable Diseases Research Center, Fasa University of Medical Sciences, Fasa, Iran


| Esmat Noshadi
Hematology Research Center, Shiraz University of Medical Sciences, Shiraz, Iran; Biotechnology Research Center, Islamic Azad University, Shahrekord Branch, Shahrekord, Iran
نشانی اینترنتی https://ijm.tums.ac.ir/index.php/ijm/article/view/3283
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